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Purified anti-IP3R1 Antibody (Previously Covance catalog# MMS-5151)

Pricing & Availability
Clone
L24/18 (See other available formats)
Regulatory Status
RUO
Other Names
Inositol 1,4,5-trisphosphate receptor type 1, IP-3-R, IP3R 1, IP3 receptor, type 1 InsP3 receptor, inositol 1,4,5-triphosphate receptor 1, inositol 1,4,5-triphosphate receptor, type 1, type 1 inositol 1,4,5-trisphosphate receptor
Previously
Covance Catalog# MMS-5151
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_L24slash18_Purified_IP3R1_Antibody_IHC_030415
IHC staining of purified anti-IP3R1 antibody (clone L24/18) on formalin fixed paraffin embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 1_L24slash18_Purified_IP3R1_Antibody_IHC_030415
    IHC staining of purified anti-IP3R1 antibody (clone L24/18) on formalin fixed paraffin embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 2_L24slash18_PURE_IP3R1_Antibody_WB_121917
    Western blot of purified anti-IP3R1 antibody (clone L24/18). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3_L24slash18_Purified_IP3R1_Antibody_IHC_2_121917
    IHC staining of purified anti-IP3R1 antibody (clone L24/18) on formalin fixed paraffin embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4_L24slash18_Purified_IP3R1_Antibody_IHC_3_121917
    IHC staining of purified anti-IP3R1 antibody (clone L24/18) on formalin fixed paraffin embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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817701 100 µL £113
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Description

Inositol 1,4,5-triphosphate (IP3) is a calcium-regulating second messenger that receptor involved in ER stress-induced apoptosis. IP3 receptor, type 1 (IP3R1) is a receptor for IP3 expressed on the ER which opens a calcium channel on the ER after IP3 bidning. IP3R1 is expressed in developing and mature neurons and its dysregulation may be involved in progression of certain neurodegenerative diseases. IP3R1 is also involved in autophagy, cellular energenetics, macrophage inflammasomes, and other cellular processes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 2680-2749 of rat IP3 receptor type 1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is less reactive in human samples than mouse and rat sample in WB.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhao G, et al. 2010. J. Gen. Physiol. 136(3):283-91 (WB)
Product Citations
  1. Watanabe S, et al. 2016. EMBO Mol Med. 8(12):1421-1437. PubMed
  2. Howie D, et al. 2019. Front Immunol. 10:1860. PubMed
RRID
AB_2564813 (BioLegend Cat. No. 817701)

Antigen Details

Structure
IP3R1 is a 2758 amino acid protein with an apparent molecular mass of 314 kD.
Distribution

Cellular distribution: Plasma membrane, endoplasmic reticulum, and nucleus.

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

Tang T-S, et al. 2009. J. Neurosci. 29 (5) 1257-66.

Gene ID
25262 View all products for this Gene ID
UniProt
View information about IP3R1 on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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