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Biotin anti-mouse/rat TNF-α Antibody

Pricing & Availability
Clone
Poly5160 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly5160_TNFa_Biotin_100411
  • Poly5160_TNFa_Biotin_100411
Cat # Size Price Quantity Check Availability Save
516003 50 µg £257
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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant mouse TNF-α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25 - 1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF-α recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

ELISA Detection: The biotinylated Poly5160 antibody is useful as a detection antibody for a sandwich ELISA when used in conjunction with purified TN3-19.12 antibody (Cat. Nos. 506102 & 506106) as the capture antibody for detecting mouse and rat TNF-α. The biotinylated Poly5160 antibody can also be used as a detection antibody in a sandwich ELISA to detect mouse TNF-α when used in conjunction with purified MP6-XT22 (Cat. Nos. 506301 & 506302) and recombinant mouse TNF-α (Cat. No. 575209) as the standard.
Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's LEGEND MAX™ Kits (Cat. Nos. 430907 & 430908) are specially developed and recommended.

Product Citations
  1. Uchiyama A, et al. 2017. J Dermatol Sci. 10.1016/j.jdermsci.2017.02.285. PubMed
  2. Allen KD, et al. 2020. Connect Tissue Res. 61:82. PubMed
  3. Schwaderer J, et al. 2020. Cell Death Dis. 11:154. PubMed
  4. Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
RRID
AB_2561411 (BioLegend Cat. No. 516003)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 18-150 kD (Mammalian).
Distribution

Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells.

Function
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists.
Interaction
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia.
Ligand/Receptor
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75).
Cell Type
B cells, Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.

Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 5    Revision Date: 12/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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