Brilliant Violet 421™ anti-human CD106 Antibody, CD106, STA

Pricing & Availability

Clone: STA (See other available formats)
Regulatory Status: RUO
Workshop: V A013
Other Names: VCAM-1, INCAM-110
Isotype: Mouse IgG1, κ
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Product Citations: publications

STA_BV421_CD106_Antibody_020320 — TNF-a stimulated HUVEC cells stained with CD106 (clone STA) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

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305815

25 tests

£148

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305816

100 tests

£298

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Description

CD106 is a 110 kD single chain type I glycoprotein also known as VCAM-1 and INCAM-110. It is expressed predominantly on activated vascular endothelium but has also been identified on follicular and interfollicular dendritic cells, some macrophages, bone marrow stromal cells, and non-vascular cell populations within joints, kidney, muscle, heart, placenta, and brain. Expression on endothelial cells as well as many other cells is induced by inflammatory stimuli and cytokines. Activated endothelial cells can release soluble forms of CD106 which can be detected in the blood. CD106 binds the integrins CD49d/CD29 (VLA-4) and α4β7 that contribute to leukocyte adhesion, transmigration, and co-stimulation of T cell proliferation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence³, immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation², ELISA² capture for sCD106, and spatial biology (IBEX)^5,6.
RRID: AB_2832595 (BioLegend Cat. No. 305815)
AB_2832595 (BioLegend Cat. No. 305816)

Antigen Details

Structure: Ig superfamily, type I glycoprotein, 110 kD
Distribution: Activated endothelial cells, endothelial progenitors, follicular dendritic cells
Function: Leukocyte adhesion, transmigration, costimulation
Ligand/Receptor: VLA-4 (CD49d/CD29)
Cell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Carlos T, et al. 1994. Blood 84:2068.
2. Jones E, et al. 1995. Nature 373:539.
Gene ID: 7412 View all products for this Gene ID
UniProt: View information about CD106 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD106 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD106	STA	FC
Biotin anti-human CD106	STA	FC
PE anti-human CD106	STA	FC,SB
PE/Cyanine5 anti-human CD106	STA	FC
Purified anti-human CD106	STA	FC,IHC-P,ICC,IP,ELISA
TotalSeq™-A0245 anti-human CD106	STA	PG
PE/Cyanine7 anti-human CD106	STA	FC
Brilliant Violet 421™ anti-human CD106	STA	FC
TotalSeq™-C0245 anti-human CD106	STA	PG
TotalSeq™-B0245 anti-human CD106	STA	PG
KIRAVIA Blue 520™ anti-human CD106	STA	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 02/03/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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