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PE/Cyanine5 anti-human CD106 Antibody

Pricing & Availability
Clone
STA (See other available formats)
Regulatory Status
RUO
Workshop
V A013
Other Names
VCAM-1, INCAM-110
Isotype
Mouse IgG1, κ
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Product Citations
publications
STA_PECy5_070507
TNF-α stimulated HUVEC cells stained with STA PE/Cyanine5
  • STA_PECy5_070507
    TNF-α stimulated HUVEC cells stained with STA PE/Cyanine5
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305808 100 tests £193
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Description

CD106 is a 110 kD single chain type I glycoprotein also known as VCAM-1 and INCAM-110. It is expressed predominantly on activated vascular endothelium but has also been identified on follicular and interfollicular dendritic cells, some macrophages, bone marrow stromal cells, and non-vascular cell populations within joints, kidney, muscle, heart, placenta, and brain. Expression on endothelial cells as well as many other cells is induced by inflammatory stimuli and cytokines. Activated endothelial cells can release soluble forms of CD106 which can be detected in the blood. CD106 binds the integrins CD49d/CD29 (VLA-4) and α4β7 that contribute to leukocyte adhesion, transmigration, and co-stimulation of T cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation2, ELISA2 capture for sCD106, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Leca G, et al. 1995. J. Immunol. 154:1069. (ELISA IP)
  3. Yen YT, et al. 2006. J. Virol. 80:2648. (IF) PubMed
  4. Dmitrieva NI, et al. 2015. PloS One.10:128870. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Appleby S, et al. 2016. J Immunol. 3302 - 3314. PubMed
  2. Mutoh T, et al. 2020. Nat Commun. 11:1253. PubMed
  3. Vanoni G, et al. 2022. Bio Protoc. 218:. PubMed
  4. Vanoni G, et al. 2021. eLife. 10:00. PubMed
RRID
AB_2214227 (BioLegend Cat. No. 305808)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 110 kD
Distribution

Activated endothelial cells, endothelial progenitors, follicular dendritic cells

Function
Leukocyte adhesion, transmigration, costimulation
Ligand/Receptor
VLA-4 (CD49d/CD29)
Cell Type
Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Carlos T, et al. 1994. Blood 84:2068.
2. Jones E, et al. 1995. Nature 373:539.

Gene ID
7412 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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