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Brilliant Violet 421™ anti-human CD96 (TACTILE) Antibody

Pricing & Availability
Clone
NK92.39 (See other available formats)
Regulatory Status
RUO
Other Names
Tactile (T cell-activated increased late expression)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NK92point39_BV421_CD96_Antibody_1_070516
Human peripheral blood lymphocytes were stained with CD19 FITC and CD96 (clone NK92.39) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • NK92point39_BV421_CD96_Antibody_1_070516
    Human peripheral blood lymphocytes were stained with CD19 FITC and CD96 (clone NK92.39) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • NK92point39_BV421_CD96_Antibody_2_070516
Compare all formats See Brilliant Violet 421™ spectral data
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338417 25 tests £121
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338418 100 tests £298
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Description

CD96, known as TACTILE (T cell-activated increased late expression), is a 160 kD type I transmembrane glycoprotein and is a member of the Ig superfamily molecules. It is expressed on NK cells, T cells (up-regulated upon activation), and some acute myeloid leukemia (AML) cells, but not on normal B and myeloid cells. A soluble form of CD96 has been found in serum. CD96 is involved in T- and NK-cell adhesion through interaction with its ligand, CD155 (polio virus receptor).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications include: block interaction of CD96 with PVR and functional assay

Application References

(PubMed link indicates BioLegend citation)
  1. Fuchs A, et al. 2004. J. Immunol. 172:3994
  2. Manes TD, et al. 2011. J. Immunol. 186:1763. PubMed
Product Citations
  1. Myers JA, et al. 2022. JCI Insight. :. PubMed
  2. Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed
RRID
AB_2629536 (BioLegend Cat. No. 338417)
AB_2629536 (BioLegend Cat. No. 338418)

Antigen Details

Structure
160 kD, type I transmembrane protein, Ig superfamily
Distribution

NK cells, T cells (upregulated upon activation)

Ligand/Receptor
CD155 (PVR)
Cell Type
NK cells, T cells
Biology Area
Immunology, Innate Immunity, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley & Sons Inc, Publication
2. Hosen N, et al. 2007. Proc. Natl. Acad. Sci. USA 104:11008
3. Gong J, et al. 2008. Clin. Exp. Immunol.

Gene ID
10225 View all products for this Gene ID
UniProt
View information about CD96 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 07/05/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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