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Brilliant Violet 421™ anti-human IL-17A Antibody

Pricing & Availability
Clone
BL168 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BL168_BV421_1_032911
Human peripheral blood lymphocytes were stimulated with PMA + Ionomycin for 6 hours (in the presence of monensin), stained with CD3 FITC, fixed, permeabilized, and then stained with IL-17A (clone BL168) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • BL168_BV421_1_032911
    Human peripheral blood lymphocytes were stimulated with PMA + Ionomycin for 6 hours (in the presence of monensin), stained with CD3 FITC, fixed, permeabilized, and then stained with IL-17A (clone BL168) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • BL168_BV421_2_032911
Compare all formats See Brilliant Violet 421™ spectral data
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512321 25 tests £148
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512322 100 tests £279
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Description

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  2. Asashima H, et al. 2022. J Clin Invest. 132: . PubMed
  3. Cao J, et al. 2023. BMC Immunol. 24:6. PubMed
  4. Imai H, et al. 2023. iScience. 26:106822. PubMed
  5. Barros-Martins J, et al. 2021. Nat Med. 27:1525. PubMed
  6. Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
  7. Zuazo M, et al. 2019. EMBO Mol Med. 11(7). PubMed
  8. Booth JS, et al. 2019. Hum Vaccin Immunother. :1. PubMed
  9. Nicholas DA et al. 2019. Cell Metab. 30(3):447-461 . PubMed
  10. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  11. Bailur JK, et al. 2020. JCI Insight. 5:00. PubMed
  12. Wood MP, et al. 2020. J Infect Dis. 222:44. PubMed
  13. Shen X, et al. 2021. Front Immunol. 12:710750. PubMed
  14. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  15. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  16. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  17. Khaitan A, et al. 2016. PLoS One. 11: 0161786. PubMed
  18. Khuzwayo S, et al. 2021. Front Immunol. 12:631410. PubMed
  19. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
RRID
AB_10899566 (BioLegend Cat. No. 512321)
AB_10899566 (BioLegend Cat. No. 512322)

Antigen Details

Structure
Cytokine; dimer; ~15 kD (Mammalian)
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
Activated T cell subpopulation
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hirota K, et al. 2007. J. Exp. Med. 204:41.
2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169.
3. Witowski J, et al. 2007. Kidney Int. 71:514.
4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255.
5. Hymowitz S, et al. 2001. EMBO J. 20:5332.

Gene ID
3605 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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