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PerCP/Cyanine5.5 anti-human IL-17A Antibody

Pricing & Availability
Clone
BL168 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BL168_PerCPCyanine55_IL-17A_Antibody_1_081424
PMA + ionomycin-stimulated (6 hours) human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) FITC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human IL-17A (clone BL168) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the lymphocyte population.
  • BL168_PerCPCyanine55_IL-17A_Antibody_1_081424
    PMA + ionomycin-stimulated (6 hours) human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) FITC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human IL-17A (clone BL168) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the lymphocyte population.
Compare all formats See PerCP/Cyanine5.5 spectral data
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512313 25 tests £141
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512314 100 tests £289
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Description

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Product Citations
  1. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  2. Rahman MA, et al. 2023. Nat Microbiol. 8:905. PubMed
  3. Lu T, et al. 2016. PLoS One. 11: 0148044. PubMed
  4. Al-Daghri N, et al. 2012. J Immunol. 188:1325. PubMed
  5. Antonelli L, et al. 2010. Blood. 116:3818. PubMed
  6. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  7. Hilchey S, et al. 2011. Blood. 118:3591. PubMed
  8. Peelen E, et al. 2013. J Neuroimmunol. 258:77. PubMed
  9. Smolders J, et al. 2010. PLoS One. 5:e15235. PubMed
  10. Dai G, et al. 2021. JCI Insight. 6:. PubMed
  11. Ferry GM, et al. 2022. Front Immunol. 13:863155. PubMed
  12. Qi Y, et al. 2012. PLoS One. 7:e39072. PubMed
  13. Tian T, et al. 2015. PLoS One. 10: 0131761. PubMed
  14. Paris R, et al. 2015. PLoS One. 10: 0144767. PubMed
RRID
AB_961397 (BioLegend Cat. No. 512313)
AB_961397 (BioLegend Cat. No. 512314)

Antigen Details

Structure
Cytokine; dimer; ~15 kD (Mammalian)
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
Activated T cell subpopulation
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hirota K, et al. 2007. J. Exp. Med. 204:41.
2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169.
3. Witowski J, et al. 2007. Kidney Int. 71:514.
4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255.
5. Hymowitz S, et al. 2001. EMBO J. 20:5332.

Gene ID
3605 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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