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Brilliant Violet 421™ anti-IκB-α Antibody

Pricing & Availability
Clone
3D6C02 (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear factor of kappa light chain enhancer in B cells-inhibitor alpha, IKBA
Isotype
Mouse IgG2b, κ
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Product Citations
publications
3D6C02_BV421_IkappaB-alpha_Antibody_021618
HeLa cells were fixed with cold 100% methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with (A) 1: 250 diluted (2 µg/ml) Brilliant Violet 421™ Mouse IgG2b, κ isotype control antibody (Cat. No. 400341) or with Brilliant Violet 421™ anti-IκB-α antibody (blue) at (B) 1: 250 (2 µg/ml) and (C) 1: 1000 (0.5 µg/ml) dilution. Nuclei were counterstained with DRAQ5 (red, Cat. No. 424101). The image was captured with a 60X objective.
  • 3D6C02_BV421_IkappaB-alpha_Antibody_021618
    HeLa cells were fixed with cold 100% methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with (A) 1: 250 diluted (2 µg/ml) Brilliant Violet 421™ Mouse IgG2b, κ isotype control antibody (Cat. No. 400341) or with Brilliant Violet 421™ anti-IκB-α antibody (blue) at (B) 1: 250 (2 µg/ml) and (C) 1: 1000 (0.5 µg/ml) dilution. Nuclei were counterstained with DRAQ5 (red, Cat. No. 424101). The image was captured with a 60X objective.
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662409 25 µg £125
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Description

IκB-α is an inhibitory protein that binds to the NF-κB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IκB proteins are phosphorylated and degraded via the ubiquitin pathway allowing for nuclear translocation of NF-κB. The NF-κB complex can then bind DNA to activate gene expression of multiple proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Hamster
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human IκB-α recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250-1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

This antibody weakly reacts with mouse and rat. Based on in-house testing, clone 3D6C02 does not block clone 25/IkBa/MAD-3, and only partially blocks L35A5 also raised against I?B-a.

RRID
AB_2728495 (BioLegend Cat. No. 662409)

Antigen Details

Structure
Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
Distribution

Cytoplasm

Function
Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
Interaction
NFkB1, SUMO4, p53, ribosomal S6 kinase 1
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Tumor Suppressors
Antigen References

1. Baeuerle PA. 1998. Cell 95:729.
2. Hoffmann A, et al. 2002. Science 298:1241.
3. Ito CY, et al. 1995. Genomics 29:490.

Gene ID
4792 View all products for this Gene ID
UniProt
View information about IkappaB-alpha on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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