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Brilliant Violet 421™ anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_BV421_062911
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 104) Brilliant Violet 421™ (filled histogram), or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 104_BV421_062911
    C57BL/6 mouse splenocytes were stained with CD45.2 (clone 104) Brilliant Violet 421™ (filled histogram), or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
109831 125 µL £125
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109832 50 µg £166
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For flow cytometric staining using the µl sizes , the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Nazet U, et al. 2021. Cells. 10:. PubMed
  2. Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed
  3. Corral D, et al. 2022. Cell Rep. 39:110715. PubMed
  4. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  5. Issa N, et al. 2023. Leukemia. :. PubMed
  6. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  7. Pulous FE, et al. 2022. Nat Neurosci. 25:567. PubMed
  8. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  9. Kinder JM, et al. 2020. Cell Reports. 31(12):107784.. PubMed
  10. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  11. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  12. Juttukonda LJ, et al. 2017. Cell Host Microbe. 22:531. PubMed
  13. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  14. Wilkinson AC, et al. 2020. Nature Protocols. 15(2):628-648.. PubMed
  15. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  16. Wiesner D, et al. 2016. J Immunol. 196: 365 - 374. PubMed
  17. Garg G et al. 2019. Cell reports. 26(7):1854-1868 . PubMed
  18. Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed
  19. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  20. Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed
  21. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  22. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  23. Crooks SD, et al. 2022. Immunohorizons. 6:705. PubMed
  24. Nazet U, et al. 2021. J Orofac Orthop. Online ahead of print. PubMed
  25. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  26. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  27. Lemaître F, et al. 2013. J Immunol. 191:1578. PubMed
  28. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
  29. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  30. Yang H, et al. 2019. J Neuroinflammation. 16:169. PubMed
  31. Zhang X, et al. 2022. JCI Insight. 7:. PubMed
  32. Severance AL, et al. 2022. iScience. 25:104400. PubMed
  33. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
  34. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  35. Engblom C, et al. 2017. Science. 358:6367. PubMed
  36. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  37. Koide S, et al. 2022. iScience. 25:103603. PubMed
  38. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  39. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  40. Senatus L, et al. 2020. JCI Insight. 5:00. PubMed
  41. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  42. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  43. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
  44. Song W, et al. 2022. Immunity. 55:290. PubMed
  45. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  46. Allman W, et al. 2015. Proc Natl Acad Sci U S A. 112: 4094 - 4103. PubMed
  47. Tribble JR, et al. 2020. Mol Brain. 13:81. PubMed
RRID
AB_10900256 (BioLegend Cat. No. 109831)
AB_10900256 (BioLegend Cat. No. 109832)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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