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Brilliant Violet 421™ anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1.4_BV421_Ly-6C_Antibody_FC_052413
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 421™ (filled histogram). Open histogram represents non-stained cells. Data shown was gated on the myeloid population.
  • HK1.4_BV421_Ly-6C_Antibody_FC_052413
    C57BL/6 mouse bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 421™ (filled histogram). Open histogram represents non-stained cells. Data shown was gated on the myeloid population.
Compare all formats See Brilliant Violet 421™ spectral data
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128031 125 µL £121
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128032 500 µL £271
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. van Dierendonck XAMH, et al. 2022. Proc Natl Acad Sci U S A. 119:e2114739119. PubMed
  2. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  3. Hindy G, et al. 2022. J Clin Invest. 132: . PubMed
  4. Zhang K, et al. 2023. Nat Metab. 5:129. PubMed
  5. Hettwer J, et al. 2022. Cardiovasc Res. 118:2778. PubMed
  6. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  7. Barberio AE, et al. 2023. Bioeng Transl Med. 8:e10453. PubMed
  8. Platt DJ, et al. 2021. Cell Reports. 35(6):109113. PubMed
  9. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  10. Rogic A, et al. 2021. Nat Commun. 12:6889. PubMed
  11. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
  12. Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
  13. Bagayoko S, et al. 2021. PLoS Pathog. 17:e1009927. PubMed
  14. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  15. Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
  16. Lu CY, et al. 2020. J Neurosci. 40:9602. PubMed
  17. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
  18. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  19. Lian J, et al. 2020. Cell Reports. 31(8):107679. PubMed
  20. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  21. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  22. Casulli J, et al. 2019. Nat Commun. 10:2121. PubMed
  23. Guichard V, et al. 2017. Elife. 6:e27215. PubMed
  24. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  25. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  26. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  27. Zhang Q, et al. 2015. Sci Rep. 5:15948. PubMed
  28. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  29. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  30. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  31. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  32. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  33. Levy N, et al. 2015. J Immunol. 195: 1713-1722. PubMed
  34. Molgora M, et al. 2020. Cell. 182:886. PubMed
  35. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  36. Tao Z, et al. 2022. Cells. 11:. PubMed
  37. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  38. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  39. Calvente CJ, et al. 2019. J Clin Invest. 130:4091. PubMed
  40. Phadke MS, et al. 2021. Cancer Immunol Res. 9:554. PubMed
  41. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  42. Kilgore AM, et al. 2020. J Immunol. 204:510. PubMed
  43. Park CJ, et al. 2020. Cell Reports. 31(2):107496.. PubMed
RRID
AB_2562177 (BioLegend Cat. No. 128031)
AB_2562177 (BioLegend Cat. No. 128032)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 12/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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