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Brilliant Violet 510™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 510™
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Product Citations
publications
SAV_BV510_Streptavidin_FC_101712
Human peripheral blood lymphocytes were stained with biotinylated CD3 (clone UCHT1) (filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram), followed by streptavidin-Brilliant Violet 510™.
  • SAV_BV510_Streptavidin_FC_101712
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (clone UCHT1) (filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram), followed by streptavidin-Brilliant Violet 510™.
See Brilliant Violet 510™ spectral data
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405233 100 µL £159
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405234 100 µg £317
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 510™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 510™ by 405 nm laser light induces a fluorescence maximum emission of 510 nm. Streptavidin-Brilliant Violet 510™ is 340 kD.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
For Cat. No. 405234: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
For Cat No. 405233: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Cat. No. 405234 is bottled at 0.5 mg/ml.
Cat. No. 405233 is bottled at 0.1 mg/ml.
Storage & Handling
The streptavidin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 510™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 510™ conjugation. For immunofluorescent staining for Cat. No. 405233, we recommend using ≤0.25 µg in 100 µl staining volume per million cells. For immunofluorescent staining for Cat. No. 405234, we recommend using ≤0.1 µg in 100 µl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The average molecular weight of Streptavidin-Brilliant Violet 510™ is 340 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
Product Citations
  1. Ho YH, et al. 2019. Cell Stem Cell. 25:407. PubMed
  2. Subas Satish HP, et al. 2022. MAbs. 14:2106621. PubMed
  3. Hettwer J, et al. 2022. Cardiovasc Res. 118:2778. PubMed
  4. Bednarski E, et al. 2022. MBio. 13:e0084022. PubMed
  5. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  6. Forte D, et al. 2020. Cell Metab. 32:829. PubMed
  7. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  8. Li Z, et al. 2022. Elife. 11:. PubMed
  9. Coppin E, et al. 2016. J Immunol. 196: 4110 - 4121. PubMed
  10. Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
  11. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  12. Wilder BK, et al. 2022. NPJ Vaccines. 7:58. PubMed
  13. Yankova E, et al. 2021. Nature. 593:597. PubMed
  14. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
  15. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  16. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  17. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  18. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
  19. Sznurkowska MK, et al. 2020. Nat Commun. 3.95625. PubMed
  20. Werner A, et al. 2021. iScience. 24:103076. PubMed
  21. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  22. Furusawa J, et al. 2016. PLoS Pathog. 12: 1005507. PubMed
  23. Dingler FA, et al. 2020. Mol Cell. . PubMed
  24. Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed
  25. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
  26. Carbonetti S et al. 2017. Journal of immunological methods. 448:66-73 . PubMed

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Go To Top Version: 4    Revision Date: 06/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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