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Brilliant Violet 605™ anti-human CD57 Recombinant Antibody

Pricing & Availability
Clone
QA17A04 (See other available formats)
Regulatory Status
RUO
Other Names
HNK-1, NK-1, Leu-7, B3GAT1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA17A04_BV650_CD56_Antibody_081018
Human peripheral blood lymphocytes were stained with CD56 APC and CD57 (clone QA17A04) Brilliant Violet 605™ (left), or mouse IgG1, κ Brilliant Violet 605™ isotype control (right).
  • QA17A04_BV650_CD56_Antibody_081018
    Human peripheral blood lymphocytes were stained with CD56 APC and CD57 (clone QA17A04) Brilliant Violet 605™ (left), or mouse IgG1, κ Brilliant Violet 605™ isotype control (right).
Compare all formats See Brilliant Violet 605™ spectral data
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393303 25 tests £133
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393304 100 tests £291
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Description

CD57, also known as HNK-1, NK-1, and Leu-7 is a 100-115 kD oligosaccharide antigenic determinant expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans. CD57 is expressed on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, and is also expressed on neural cells and striated muscle. CD57 is not expressed on red blood cells, granulocytes, monocytes, or platelets. While the function of CD57 is unknown, binding to L-selectin, P-selectin, and a fragment of laminin suggests that CD57 may be involved in cell-matrix interactions. CD57 is increased in some disease states associated with CD4/CD8 imbalances (AIDS, autoimmune disease, viral infections, and allograft transplants).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Membrane extract of human lymphoblastoid cell line HSB-2.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Scheer I, et al. 2023. Cells. 12:. PubMed
  2. Au L, et al. 2021. Cancer Cell. 39:1497. PubMed
  3. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
  4. Gatla H, et al. 2022. Front Med Technol. 4:850565. PubMed
  5. Breen EC, et al. 2022. iScience. 25:104488. PubMed
  6. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  7. Mold JE, et al. 2021. Cell Reports. 35(8):109174. PubMed
  8. Meziane O, et al. 2020. J Virol. 94:00:00. PubMed
RRID
AB_2728425 (BioLegend Cat. No. 393303)
AB_2728425 (BioLegend Cat. No. 393304)

Antigen Details

Structure
Oligosaccharide antigenic determinant present on a variety of proteins, lipds, and chrondroitin sulfate proteoglycans, 100-115 kD. The antigen is convserved across species.
Distribution

NK cells, subset of CD8+ T cells, neural cells, striated muscle

Ligand/Receptor
Binds to L-selectin and P-selectin in a calcium-dependent manner, also binds to second globular domain of E8 laminin fragment.
Cell Type
NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711-714.
  2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
  3. Schachner M, et al. 1995. Trends Neurosci. 18:183.
  4. Wood KL, et al. 2005. Clin. Immunol. 117:294.
Gene ID
27087 View all products for this Gene ID
UniProt
View information about CD57 on UniProt.org
Go To Top Version: 1    Revision Date: 02/15/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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