CD4 Fluorophore Sampler Kit with Veri-Cells PBMC

Pricing & Availability

Clone: RPA-T4 (See other available formats)
Regulatory Status: RUO
Other Names: T4, staining index, spillover spreading
Isotype: Mouse IgG1, κ
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Product Citations: publications

CD4_Flurophore_Samplet_Kit_1_121217 — Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA-T4) in each fluorophore conjugate separately.

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300562

1 kit

£722

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Description

The CD4 Fluorophore Sampler Kit contains 18 different fluorophore formats of clone RPA-T4 with 10 tests of each antibody per vial. It can be useful to have the same clone in multiple fluorophore formats to enable instrument characterization and standardization. This kit also contains a vial of 25 tests of Veri-Cells™ PBMC.

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Brilliant Violet™ conjugates: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
All other conjugates: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Storage & Handling: The kit should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Brilliant Violet 421™, Brilliant Violet 510™, Brilliant Violet 570™, Brilliant Violet 605™, Brilliant Violet 650™, Brilliant Violet 711™, Brilliant Violet 785™ are trademarks of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Red Laser (633 nm)
Application Notes: The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections^3,4,5, and blocking of T cell activation^1,2. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300516).
RRID: AB_3097054 (BioLegend Cat. No. 300562)

Antigen Details

Cell Type: Dendritic cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Gene ID: 920 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

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Related Protocols

Protocol for using Veri-Cells™

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Related Pages & Pathways

Pathways

Related FAQs

Is washing required after staining?: Yes, it is recommended to wash after staining. We recommend using BioLegend's Cell Staining buffer (Cat. No. 420201) for the wash step.
I am unable to see expression of T cell markers such as CD3 and CD4 post activation.: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Can the cells be activated after rehydration?: No, they cannot be activated.
Are these cells virus/pathogen free?: These are tested to be free of HIV, HBV, syphilis, and HCV.
Are there any RBCs in Veri-cells™?: There may be a few, but the majority of RBCs are not present in Veri-Cells™ preparations.
Are these cells viable?: No they are not.
Can a constant percentage of each population be expected in each lot?: Yes, within each lot, cell populations remain constant.
Are the cells fixed?: They are treated with our proprietary mixture prior to lyophilization.
Can they be used to detect intracellular cytokines such as TNF-α or IFN-γ?: For this, use Veri-Cells™ Activated (Cytokine) PBMC which have been activated with Cell Activation Cocktail (with Brefeldin A) containing PMA and Ionomycin. These have been validated to stain positive for activation-induced cytokines including TNF-α and IFN-γ. Veri-Cells™ PBMC and Veri-Cells™ CD4-Low PBMC will not have detectable TNF-α or IFN-γ.
How do we QC these cells?: Each Veri-Cells™ product is tested with a panel of antibodies to ensure expression of these markers falls within the expected range. For more information regarding the exact markers used for each product, please contact technical service.

Other Formats

View All CD4 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD4	RPA-T4	FC
Biotin anti-human CD4	RPA-T4	FC
FITC anti-human CD4	RPA-T4	FC,SB
PE anti-human CD4	RPA-T4	FC
PE/Cyanine5 anti-human CD4	RPA-T4	FC
PE/Cyanine7 anti-human CD4	RPA-T4	FC
Purified anti-human CD4	RPA-T4	FC,CyTOF®,Activ,Block,IHC
APC/Cyanine7 anti-human CD4	RPA-T4	FC
Alexa Fluor® 488 anti-human CD4	RPA-T4	FC,ICC
Alexa Fluor® 647 anti-human CD4	RPA-T4	FC,SB
Pacific Blue™ anti-human CD4	RPA-T4	FC
Brilliant Violet 421™ anti-human CD4	RPA-T4	FC,ICC
Alexa Fluor® 700 anti-human CD4	RPA-T4	FC,SB
PerCP anti-human CD4	RPA-T4	FC
PerCP/Cyanine5.5 anti-human CD4	RPA-T4	FC
Brilliant Violet 570™ anti-human CD4	RPA-T4	FC
Brilliant Violet 650™ anti-human CD4	RPA-T4	FC
Purified anti-human CD4 (Maxpar® Ready)	RPA-T4	FC,CyTOF®
Alexa Fluor® 594 anti-human CD4	RPA-T4	FC,ICC
Brilliant Violet 510™ anti-human CD4	RPA-T4	FC
PE/Dazzle™ 594 anti-human CD4	RPA-T4	FC
Brilliant Violet 785™ anti-human CD4	RPA-T4	FC
Brilliant Violet 605™ anti-human CD4	RPA-T4	FC
Brilliant Violet 711™ anti-human CD4	RPA-T4	FC
APC/Fire™ 750 anti-human CD4	RPA-T4	FC
CD4 Fluorophore Sampler Kit	RPA-T4	FC
CD4 Fluorophore Sampler Kit with Veri-Cells™ PBMC	RPA-T4	FC
TotalSeq™-A0072 anti-human CD4	RPA-T4	PG
TotalSeq™-B0072 anti-human CD4	RPA-T4	PG
TotalSeq™-C0072 anti-human CD4	RPA-T4	PG
Ultra-LEAF™ Purified anti-human CD4	RPA-T4	FC,CyTOF®,Activ,Block,IHC
TotalSeq™-D0072 anti-human CD4	RPA-T4	PG

Certificate of Analysis

Go To Top Version: 3 Revision Date: 11/08/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.