TotalSeq™-D0072 anti-human CD4 antibody, CD4, RPA-T4

Pricing & Availability

Clone: RPA-T4 (See other available formats)
Regulatory Status: RUO
Workshop: IV T114
Other Names: T4
Isotype: Mouse IgG1, κ
Barcode Sequence: TGTTCCCGCTCAACT
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Product Citations: publications

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300575

10 µg

£253

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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes: The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections^3,4,5, blocking of T cell activation^1,2, and spatial biology (IBEX)^10,11. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
Additional Product Notes: TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.
RRID: AB_2892343 (BioLegend Cat. No. 300575)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

Documentation

Reviews

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Related Protocols

TotalSeq™-D with Mission Bio Tapestri® Single-Cell DNA + Protein Protocol

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Related Pages & Pathways

Pathways

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD4	RPA-T4	FC
Biotin anti-human CD4	RPA-T4	FC
FITC anti-human CD4	RPA-T4	FC,SB
PE anti-human CD4	RPA-T4	FC
PE/Cyanine5 anti-human CD4	RPA-T4	FC
PE/Cyanine7 anti-human CD4	RPA-T4	FC
Purified anti-human CD4	RPA-T4	FC,CyTOF®,Activ,Block,IHC
APC/Cyanine7 anti-human CD4	RPA-T4	FC
Alexa Fluor® 488 anti-human CD4	RPA-T4	FC,ICC
Alexa Fluor® 647 anti-human CD4	RPA-T4	FC,SB
Pacific Blue™ anti-human CD4	RPA-T4	FC
Brilliant Violet 421™ anti-human CD4	RPA-T4	FC,ICC
Alexa Fluor® 700 anti-human CD4	RPA-T4	FC,SB
PerCP anti-human CD4	RPA-T4	FC
PerCP/Cyanine5.5 anti-human CD4	RPA-T4	FC
Brilliant Violet 570™ anti-human CD4	RPA-T4	FC
Brilliant Violet 650™ anti-human CD4	RPA-T4	FC
Purified anti-human CD4 (Maxpar® Ready)	RPA-T4	FC,CyTOF®
Alexa Fluor® 594 anti-human CD4	RPA-T4	FC,ICC
Brilliant Violet 510™ anti-human CD4	RPA-T4	FC
PE/Dazzle™ 594 anti-human CD4	RPA-T4	FC
Brilliant Violet 785™ anti-human CD4	RPA-T4	FC
Brilliant Violet 605™ anti-human CD4	RPA-T4	FC
Brilliant Violet 711™ anti-human CD4	RPA-T4	FC
APC/Fire™ 750 anti-human CD4	RPA-T4	FC
CD4 Fluorophore Sampler Kit	RPA-T4	FC
CD4 Fluorophore Sampler Kit with Veri-Cells™ PBMC	RPA-T4	FC
TotalSeq™-A0072 anti-human CD4	RPA-T4	PG
TotalSeq™-B0072 anti-human CD4	RPA-T4	PG
TotalSeq™-C0072 anti-human CD4	RPA-T4	PG
Ultra-LEAF™ Purified anti-human CD4	RPA-T4	FC,CyTOF®,Activ,Block,IHC
TotalSeq™-D0072 anti-human CD4	RPA-T4	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.