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Cyanine3 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Cyanine3, SAV
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Product Citations
publications
See Cyanine3 spectral data
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405215 100 µg £81
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Cyanine3 is useful for detecting biotinylated antibodies. The excitation of Cyanine3 by 550 nm laser light induces a light emission maximum of 570 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with Cyanine3 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-Cyanine3 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F, IHC-P
Recommended Usage

It is recommended that the reagent be titrated for optimal performance for each application. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Cyanine3 conjugation.

Application Notes

The average molecular weight of Streptavidin-Cyanine3 is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Heidler J, et al. 2013. Dis Model Mech. 6:1378. PubMed
  2. Thoma CR, et al. 2013. J Biomol Screen. 18:1330. PubMed
  3. Sunshine J, et al. 2014. J. Virol. 88:1354. PubMed
  4. Kai D, et al. 2014. Acta Biomater. 10:2727. PubMed
  5. Silginer M, et al. 2014. Cell Death Dis. 5:1012. PubMed
Product Citations
  1. Franjic D, et al. 2022. Neuron. 110:452. PubMed
  2. Kai D, et al. 2014. Acta Biomater. 10:2727. PubMed
  3. Cacialli P, et al. 2022. EMBO J. 41:e108536. PubMed
  4. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  5. Atreya I, et al. 2016. J Crohns Colitis. 10: 1132 - 1143. PubMed
  6. Kelm W 2013. J Biomol Screen. 18:1330. PubMed
  7. Heidler J, et al. 2013. Dis Model Mech. 6:1378. PubMed
  8. Silginer M, et al. 2014. Cell Death Dis. 5:1012. PubMed
  9. Sunshine J, et al. 2014. J Virol. 88:1354. PubMed
  10. Fukuyama T, et al. 2015. J Pharmacol Exp Ther. 354: 394 - 405. PubMed
  11. Sharma N, et al. 2015. J Immunol. 195: 5227 - 5236. PubMed
  12. Okamura Y, et al. 2017. Allergol Int. 10.1016/j.alit.2017.03.002. PubMed

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Go To Top Version: 6    Revision Date: 09/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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