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Cyanine5 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Cyanine5, SAV
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Product Citations
publications
FrancescaMazzoni.jpg
Unpassaged live mouse primary retinal pigment epithelial cells engulfed biotinylated photocreceptor outer segments (POS) coupled to Cyanine5 Streptavidin (red). Cells were subsequently stained with Flash Phalloidin™ Green 488 (green) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Francesca Mazzoni from Fordham University.
  • FrancescaMazzoni.jpg
    Unpassaged live mouse primary retinal pigment epithelial cells engulfed biotinylated photocreceptor outer segments (POS) coupled to Cyanine5 Streptavidin (red). Cells were subsequently stained with Flash Phalloidin™ Green 488 (green) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Francesca Mazzoni from Fordham University.
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405209 100 µg £141
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Cyanine5 is useful for detecting biotinylated antibodies. The excitation of Cyanine5 by 625-650 nm laser light induces a fluorescence emission maximum of 670 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with Cyanine5 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-Cyanine5 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-Cyanine5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Cyanine5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.5 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Streptavidin-Cyanine5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of streptavidin-Cyanine5 is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu HC, et al. 2007. J. Immunol. 178:5357.
  2. Biegler KA, et al. 2012. Cancer Prev Res (Phila). 5:1173. PubMed
  3. Cheng JY, et al. 2014. Cancer Res. 74:6856. PubMed
Product Citations
  1. Garrison MA, et al. 2023. Sci Data. 813:10. PubMed
  2. Müller-Durovic B, et al. 2016. J Immunol. 197: 2891 - 2899. PubMed
  3. Cheng J, et al. 2014. Cancer Res. 74:6856. PubMed
  4. Dedeoglu B, et al. 2016. PLoS One. 11: 0150826. PubMed
  5. Biegler K, et al. 2012. Cancer Prev Res. 5:1173. PubMed

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Go To Top Version: 5    Revision Date: 09/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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