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Direct-Blot™ HRP anti-GAPDH Antibody

Pricing & Availability
Clone
FF26A/F9 (See other available formats)
Regulatory Status
RUO
Other Names
glyceraldehyde-3-phosphate dehydrogenase
Isotype
Mouse IgG1, κ
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Product Citations
publications
FF26AslashF9_DirectBlotHRP_GAPDH_WB_Image_010616
15 µg of total protein extract from HeLa cells was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-GAPDH (clone FF26A/F9 ). Proteins were visualized using chemiluminescence detection.
  • FF26AslashF9_DirectBlotHRP_GAPDH_WB_Image_010616
    15 µg of total protein extract from HeLa cells was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-GAPDH (clone FF26A/F9 ). Proteins were visualized using chemiluminescence detection.
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649204 25 µL £70
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649203 100 µL £233
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Description

This mouse monoclonal GAPDH antibody recognizes human GAPDH, also known as glyceraldehyde-3-phosphate dehydrogenase. GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases. The FF26A/F9 monoclonal antibody has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD4 lymphocytes
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

The optimal concentration should be determined by titration for each individual assay of interest.
Clone FF26A/F9 is only reactive to human GAPDH, and not cross-reactive to mouse or rat GAPDH.

Application References

(PubMed link indicates BioLegend citation)
  1. Maestre L, et al. 2009. Haematologica. 94:419.
  2. Zou L, et al. 2012. J Biol. Chem. 287:7190. PubMed
  3. Chen CY, et al. 2012. PNAS. 110:630 PubMed
  4. Trifari S, et al. 2013. PNAS. PubMed
  5. Liu CC, et al. 2013. Mol. Cell Biol. 33:4334. PubMed
  6. Trifari S, et al. 2013. PNAS. 110:18608. PubMed
  7. Butin-Israeli V, et al. 2015. Mol. Cell Biol. 35:884. PubMed
Product Citations
  1. Gholam MF, et al. 2023. Int J Mol Sci. 24: . PubMed
  2. Jiang D, et al. 2023. Nat Commun. 14:3642. PubMed
  3. Kyle Cottrell, Sergej Djuranovic 2016. PLoS One. 11:e0167877. PubMed
  4. Wang Z, et al. 2019. Sci Rep. 9:1853. PubMed
  5. Powell G, et al. 2021. Mol Ther Nucleic Acids. 26:865. PubMed
  6. Zu T, et al. 2021. Lab Invest. 101:636. PubMed
RRID
AB_2566698 (BioLegend Cat. No. 649204)
AB_2566698 (BioLegend Cat. No. 649203)

Antigen Details

Structure
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution

Ubiquitously expressed, locate in cytoplasm and perinuclear region.

Function
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and and nicotinamide adenine dinucleotide (NAD). Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretion pathway.
Interaction
Interacts with TPPP. Interacts with EIF1ADand WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Signal Transduction, Western Blot Controls
Antigen References

1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
2. Meyer-Siegler K, et al. 1991. P. Natl. Acad. Sci. USA 88:8460.
3. Hara MR and Snyder SH. 2006. Cell Mol. Neurobiol. 26:527.
4. Zheng L, et al. 2003. Cell. 114:255.
5. Wang Q, et al. 2005. FASEB J. 19:869.
6. Bae BI, et al. 2006. P. Natl. Acad. Sci. USA. 103:3405.

Gene ID
5590 View all products for this Gene ID
UniProt
View information about GAPDH on UniProt.org
Go To Top Version: 5    Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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