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Purified anti-GAPDH Antibody

Pricing & Availability
Clone
FF26A/F9 (See other available formats)
Regulatory Status
RUO
Other Names
glyceraldehyde-3-phosphate dehydrogenase
Isotype
Mouse IgG1, κ
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Product Citations
publications
FF26A-F9_Purified_GAPDH_Antibody_031919
Whole cell extracts (15 µg protein) from HeLa cells were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-GAPDH Antibody, clone FF26A/F9, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular Weight marker.
  • FF26A-F9_Purified_GAPDH_Antibody_031919
    Whole cell extracts (15 µg protein) from HeLa cells were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-GAPDH Antibody, clone FF26A/F9, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular Weight marker.
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649201 25 µg £85
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649202 100 µg £157
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Description

This mouse monoclonal GAPDH antibody recognizes human GAPDH, also known as glyceraldehyde-3-phosphate dehydrogenase. GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases. The FF26A/F9 monoclonal antibody has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD4 lymphocytes
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC, IP - Reported by the developer, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1 - 1.0 µg antibody per 1 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The optimal concentration should be determined by titration for each individual assay of interest.
Clone FF26A/F9 is only reactive to human GAPDH, and not cross-reactive to mouse or rat GAPDH.

Application References

(PubMed link indicates BioLegend citation)
  1. Maestre L, et al. 2009. Haematologica. 94:419.
  2. Zou L, et al. 2012. J Biol. Chem. 287:7190. PubMed
  3. Chen CY, et al. 2012. PNAS. 110:630 PubMed
  4. Trifari S, et al. 2013. PNAS. PubMed
  5. Liu CC, et al. 2013. Mol. Cell Biol. 33:4334. PubMed
  6. Trifari S, et al. 2013. PNAS. 110:18608. PubMed
  7. Butin-Israeli V, et al. 2015. Mol. Cell Biol. 35:884. PubMed
Product Citations
  1. Trifari S, et al. 2013. Proc Natl Acad Sci U S A. 110:18608. PubMed
  2. Hsu J, et al. 2022. J Biol Chem. 299:102848. PubMed
  3. Strine MS, et al. 2023. PLoS Biol. 21:e3002097. PubMed
  4. Wahlig S, et al. 2020. Sci Rep. 1.584027778. PubMed
  5. Ong SM, et al. 2018. Cell Death Dis. 9:266. PubMed
  6. Zeng L, et al. 2021. J Cell Biol. 220:. PubMed
  7. Butin-Israeli V, et al. 2015. Mol Cell Biol. 35:884. PubMed
  8. Ruehle S et al. 2017. Journal of neurochemistry. 142(4):521-533 . PubMed
  9. Zou L, et al. 2012. J Biol Chem. 287:7190. PubMed
  10. Liu R, et al. 2021. Oncol Lett. 21:06. PubMed
  11. Liu C, et al. 2013. Mol Cell Biol. 33:4334. PubMed
  12. Betriu N, et al. 2021. Cancers (Basel). 13:. PubMed
  13. Gorji-Bahri G, et al. 2021. J Cell Biochem. 122:425. PubMed
  14. Lovatt M, et al. 2018. Antioxidants (Basel). 0.416666667. PubMed
  15. Barbosa JAF, et al. 2021. J Virol. 95:e0097120. PubMed
  16. Chen C, et al. 2013. Proc Natl Acad Sci U S A. 110:630. PubMed
  17. Zhao J et al. 2019. Transl Psychiatry. 9(1):247 . PubMed
  18. Dragoj M, et al. 2017. Invest New Drugs. 10.1007/s10637-017-0494-4. PubMed
RRID
AB_10612752 (BioLegend Cat. No. 649201)
AB_10612752 (BioLegend Cat. No. 649202)

Antigen Details

Structure
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution

Ubiquitously expressed, locate in cytoplasm and perinuclear region.

Function
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and and nicotinamide adenine dinucleotide (NAD). Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretion pathway.
Interaction
Interacts with TPPP. Interacts with EIF1ADand WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Signal Transduction
Antigen References

1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
2. Meyer-Siegler K, et al. 1991. P. Natl. Acad. Sci. USA 88:8460.
3. Hara MR and Snyder SH. 2006. Cell Mol. Neurobiol. 26:527.
4. Zheng L, et al. 2003. Cell. 114:255.
5. Wang Q, et al. 2005. FASEB J. 19:869.
6. Bae BI, et al. 2006. P. Natl. Acad. Sci. USA. 103:3405.

Gene ID
5590 View all products for this Gene ID
UniProt
View information about GAPDH on UniProt.org
Go To Top Version: 5    Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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