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- Regulatory Status
- RUO
- Other Names
- Human Aβ42, Amyloid-beta protein 42
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 448704 | 5 plates | £271 | ||||
The Amyloid peptides are peptides of 36-43 amino acids which are produced through the post-translational cleavage of the Amyloid Precursor Protein by beta and gamma secretase. Although numerous different Amyloid-Beta species exist, the majority of the Amyloid-Beta peptides produced by post-translational modification consists of either Amyloid-Beta (1-40) (80-90%) and Amyloid-Beta (1-42) (5-10%). Both variants are major components of the extracellular plaques found in Alzheimer’s brain. However, Amyloid-Beta (1-42) is reported to be more prone to aggregation than the (1-40) variant despite differing in only 2 amino acids at the C terminus. Other studies that aimed at characterizing the difference in aggregation tendency between the two variants suggested that they have distinct 3D structures in solution.
BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The kit contains a monoclonal capture antibody and biotinylated monoclonal antibody specific for human Amyloid-Beta (1-42). This kit is specifically designed for the accurate quantitation of human Amyloid-Beta (1-42) in cerebrospinal fluid, serum and plasma. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.
Kit Contents
- Kit Contents
-
- Human Amyloid- Beta (1-42) Capture Antibody (200X)
- Human Amyloid- Beta (1-42) Detection Antibody (200X)
- Human Amyloid-Beta (1-42) Lyophilized Standard
- Streptavidin-Polymer HRP (1,000X)
- Coating Buffer B (5X)
- Blocking Buffer A (2X)
- ELISA Diluent 1 (20X)
- Substrate Solution D
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 0.718 ± 0.190 pg/mL
- Standard Range
- 3.125 - 200 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- Neural Stem Cells, Neurons
- Biology Area
- Cell Death, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation
- Molecular Family
- APP/β-Amyloid, APP/Aβ Degradation
- Gene ID
- NA
- UniProt
- View information about Amyloid Beta 1-42 on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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