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FITC anti-human S100A4 Antibody

Pricing & Availability
Clone
NJ-4F3-D1 (See other available formats)
Regulatory Status
RUO
Other Names
S100 Calcium Binding Protein A4, 42A, 18A2, CAPL, FSP1, MTS1, P9KA, PEL98, Calvasculin, Metastasin, Placental calcium-binding protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NJdash4F3dashD1_FITC_S100A4_Antibody_032316
Human peripheral blood mononuclear cells were fixed and permeabilized. Cells were then stained with S100A4 FITC (clone NJ-4F3-D1) (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram). Data shown was gated on the monocyte population.
  • NJdash4F3dashD1_FITC_S100A4_Antibody_032316
    Human peripheral blood mononuclear cells were fixed and permeabilized. Cells were then stained with S100A4 FITC (clone NJ-4F3-D1) (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram). Data shown was gated on the monocyte population.
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370007 25 tests £101
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370008 100 tests £238
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Description

S100A4 is a member of the S100 family of calcium-binding proteins that contain two Ca2+-binding EF-hand motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It can be secreted by unknown mechanisms, stimulating a variety of cellular responses, including angiogenesis and neuronal growth. S100A4 has been shown to be a prognostic marker in a number of human cancers. It also has a potential use as a marker for tumor metastasis and disease progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
cDNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA Detection.

Application References

(PubMed link indicates BioLegend citation)
  1. Eisenbacher JL, et al. 2014. J. Immunol. 192:6102.
Product Citations
  1. Viswanathan G, et al. 2023. Am J Respir Crit Care Med. 1358:207. PubMed
  2. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
RRID
AB_2572073 (BioLegend Cat. No. 370007)
AB_2572073 (BioLegend Cat. No. 370008)

Antigen Details

Structure
EF-hand motif.
Distribution
Widely expressed; localized in the cytoplasm and/or nucleus of a vast range of cells.
Function
Calcium-binding.
Cell Type
Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Immune Checkpoint Receptors
Antigen References

1. Schneider M, et al. 2008. J. Mol. Med. 86:507.
2. Donato R, et al. 2013. Curr. Mol. Med. 13:24.
3. Sherbet GV. 2009. Cancer Lett. 280:15.

Gene ID
6275 View all products for this Gene ID
UniProt
View information about S100A4 on UniProt.org

Other Formats

View All S100A4 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-human S100A4 NJ-4F3-D1 ICFC
Purified anti-human S100A4 NJ-4F3-D1 ICFC,ELISA Detection
FITC anti-human S100A4 NJ-4F3-D1 ICFC
PerCP/Cyanine5.5 anti-human S100A4 NJ-4F3-D1 ICFC
APC anti-human S100A4 NJ-4F3-D1 ICFC
Go To Top Version: 1    Revision Date: 03/24/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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