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GMP Ultra-LEAF™ Biotin anti-human CD3 SF Antibody

Pricing & Availability
Clone
OKT3 (See other available formats)
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
OKT3_GMP_ULEAF_BIOTIN_CD3_1_052523
Human peripheral lymphocytes were stained with GMP Ultra-LEAF™ Biotin anti-human CD3 SF antibody (clone OKT3) (filled histogram) or isotype control (clone MOP-173) (open histogram), followed by Streptavidin-PE stain.
  • OKT3_GMP_ULEAF_BIOTIN_CD3_1_052523
    Human peripheral lymphocytes were stained with GMP Ultra-LEAF™ Biotin anti-human CD3 SF antibody (clone OKT3) (filled histogram) or isotype control (clone MOP-173) (open histogram), followed by Streptavidin-PE stain.
  • OKT3_GMP_ULEAF_BIOTIN_CD3_2_052523
    PBMC-derived T cells were activated in the presence of GMP Ultra-LEAF™ Biotin anti-human CD28 SF antibody (Cat. No. 302978) alone, GMP Ultra-LEAF™ Biotin anti-human CD3 SF antibody (Cat. No. 317358) alone, or the combination of both antibodies (Cat. No. 302978 and 317358) for a period of 4 days. Activation was done via plate coating, with 1 µg/mL of each antibody. Cells were cultured for a total of 11 days and viable cell number was determined
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317357 100 µg £396
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317358 1 mg £2032
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Description

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

GMP Ultra-LEAF™ Biotin anti-human CD3 SF Antibody was GMP manufactured under serum-free conditions including serumfree hybridoma cell culture, without additional animal or humanderived materials or preservatives. This antibody contains ultra-low levels of endotoxin (<0.01EU/μg of protein), is filtered through a 0.1 μm membrane, and is tested negative for mycoplasma and microbial growths.

GMP Ultra-LEAF™ Biotin anti-human CD3 SF Antibody is for research and further ex vivo bioprocessing use only.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.1 μm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

GMP Ultra-LEAF™ Biotin anti-human CD3 SF Antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.1 μm filtered) is recommended for highly sensitive assays. The antibody was GMP manufactured under serum-free conditions including serum-free hybridoma cell culture, without additional animal or human-derived materials or preservatives. This antibody is intended for flow cytometry and for research or further ex vivo bioprocessing use only.

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. Clone OKT3 can block the binding of clones SK7 and UCHT14. The OKT3 antibody is able to induce T cell activation.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells.

Additional Product Notes

For plate coating protocols, Biotin anti-human CD3 antibody (Cat. No. 317357/317358) can be used at 1 μg/mL in PBS in combination with 1 μg/mL of Biotin anti-human CD28 antibody (Cat. No. 302978) to efficiently activate T cells. Plates should be coated for 2 hours in a tissue culture incubator or overnight at 4°C. For soluble antibody activation protocols, we recommend 10 μg/mL of anti-human CD3 (Cat. No. 317357/317358) antibody and 5 μg/mL of anti-human CD28 antibody (Cat. No. 302978).

Disclaimer

GMP Ultra-LEAF™ antibodies. BioLegend GMP Ultra-LEAF™ antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency    
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

BioLegend GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 k
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.

Beverly P, et al. 1981. Eur. J. Immunol. 11:329.

Lanier L, et al. 1986. J. Immunol. 137:2501.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 06/12/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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