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Brilliant Violet 650™ anti-human CD3 Antibody

Pricing & Availability
Clone
OKT3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
OKT3_BV650_020312
Human peripheral lymphocytes were stained with CD3 (clone OKT3) Brilliant Violet 650™.
  • OKT3_BV650_020312
    Human peripheral lymphocytes were stained with CD3 (clone OKT3) Brilliant Violet 650™.
Compare all formats See Brilliant Violet 650™ spectral data
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317323 25 tests £148
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317324 100 tests £286
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Description

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Li B, et al. 2005. Immunology 116:487.
  5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
  6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
  7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
  8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
  9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations
  1. Cox MJ, et al. 2022. Leukemia. 36:1635. PubMed
  2. Keeton R 2023. Cell Reports Medicine. 4(1):100898. PubMed
  3. Touizer E, et al. 2023. iScience. 26:105862. PubMed
  4. Du Bruyn E, et al. 2023. Nat Commun. 14:188. PubMed
  5. Consonni FM, et al. 2023. Front Immunol. 14:1168455. PubMed
  6. Lucera M, et al. 2014. J Virol. 88:10803. PubMed
  7. Savage TM, et al. 2018. JCI Insight. 3:. PubMed
  8. Riou C, et al. 2014. PLoS One. 9:102178. PubMed
  9. Xu Y, et al. 2015. J Vis Exp. 99: 52863. PubMed
  10. Boyle M, et al. 2015. PLoS Pathog. 11: 1005041. PubMed
  11. Booth JS, et al. 2019. Hum Vaccin Immunother. :1. PubMed
  12. Boyle M, et al. 2015. J Infect Dis. 212: 416-425. PubMed
  13. Naaber P, et al. 2022. Cell Rep Med. 3:100716. PubMed
  14. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  15. Mikulak J, et al. 2019. JCI Insight. 4:e125884. PubMed
  16. Tilton C, et al. 2013. J Virol Methods. 166:376. PubMed
  17. Lu Y, et al. 2021. Gastroenterology. 161:575. PubMed
  18. Keeton R, et al. 2021. Cell Host Microbe. 29:1611. PubMed
  19. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  20. Vardam-Kaur T, et al. 2021. Oncotarget. 12:2051. PubMed
  21. Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed
  22. Hardman CS, et al. 2021. Sci Immunol. 6:. PubMed
  23. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  24. Poon MML, et al. 2021. Cell Rep. 37:110071. PubMed
  25. Escudero–Pérez B, et al. 2019. JCI Insight. 4:e126070. PubMed
  26. Khosravi-Maharlooei M, et al. 2021. J Autoimmun. 119:102612. PubMed
  27. Franco LM, et al. 2019. J Exp Med. 216:384. PubMed
  28. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  29. Khuzwayo S, et al. 2021. Front Immunol. 12:631410. PubMed
  30. Boudreau J, et al. 2014. PLoS One. 9:99543. PubMed
  31. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  32. Mattiola I, et al. 2015. J Immunol. 195: 2818-2828. PubMed
  33. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  34. Querfeld C, et al. 2018. Cancer Immunol Res. 6:900. PubMed
  35. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  36. Pierce CA, et al. 2020. Sci Transl Med. 12:00. PubMed
  37. Robert R, et al. 2017. PLoS One. 10.1371/journal.pone.0184278. PubMed
  38. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  39. Salumets A, et al. 2022. Aging Cell. 21:e13607. PubMed
  40. Garfall AL, et al. 2018. JCI Insight. 3. PubMed
RRID
AB_11126748 (BioLegend Cat. No. 317323)
AB_11126748 (BioLegend Cat. No. 317324)

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
  2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
  3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 2    Revision Date: 06/12/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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