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Pacific Blue™ anti-human Siglec-9 Antibody

Pricing & Availability
Clone
K8 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid-binding Ig-like lectin-9, CD329
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
K8_PB_Siglec-9_Antibody_122717
Human peripheral blood granulocytes were stained with Siglec-9 (clone K8) PE (filled histogram) or mouse IgG1 Pacific Blue™ isotype control (open histogram).
  • K8_PB_Siglec-9_Antibody_122717
    Human peripheral blood granulocytes were stained with Siglec-9 (clone K8) PE (filled histogram) or mouse IgG1 Pacific Blue™ isotype control (open histogram).
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Cat # Size Price Quantity Check Availability Save
351507 25 tests £105
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351508 100 tests £238
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Description

Siglecs are cell surface receptors belonging to the immunoglobulin superfamily that recognize sugar antigens. The extracellular domain of siglec-9 contains an IgV region, which binds sialic acid, followed by two IgC regions. Siglec 9 and siglec 6-8,10-12 are CD33 (siglec 3) like siglecs, which have two ITIMs in the cytoplasmic tails, suggesting their functional involvement in signal transduction. It is highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T and B lymphocytes and NK cells. Siglec-9 plays a role in negative regulation of T cell activation, and it also affects neutrophil apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-9 fused to Fc region of human IgG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence staining2, Western blotting2, immunoprecipitation2, and ELISA3.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhang J, et al. 2000. J. Biol. Chem. 275:22121.
  2. Avril T, et al. 2004. J. Immunol. 173:6841. (IF, IP, WB)
  3. Biedermann B, et al. 2007. Leukemia Res. 31:221. (ELISA)
RRID
AB_2721616 (BioLegend Cat. No. 351507)
AB_2721616 (BioLegend Cat. No. 351508)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T cells and B lymphocytes and NK cells

Ligand/Receptor
Sialic acid
Cell Type
Monocytes, Neutrophils
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Ikehara Y, et. al. 2004. J. Biol. Chem. 279:43117.
2. von Gunten S, et al. 2005. Blood 106:1423.

Gene ID
27180 View all products for this Gene ID
UniProt
View information about Siglec-9 on UniProt.org
Go To Top Version: 1    Revision Date: 12/27/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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