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Purified anti-human Siglec-9 Antibody

Pricing & Availability
Clone
K8 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid-binding Ig-like lectin-9, CD329
Isotype
Mouse IgG1, κ
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Product Citations
publications
K8_Pure_062311
Human peripheral blood granulocytes were stained with purified Siglec-9 (clone K8) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • K8_Pure_062311
    Human peripheral blood granulocytes were stained with purified Siglec-9 (clone K8) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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351502 100 µg £157
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Description

Siglecs are cell surface receptors belonging to the immunoglobulin superfamily that recognize sugar antigens. The extracellular domain of siglec-9 contains an IgV region, which binds sialic acid, followed by two IgC regions. Siglec 9 and siglec 6-8,10-12 are CD33 (siglec 3) like siglecs, which have two ITIMs in the cytoplasmic tails, suggesting their functional involvement in signal transduction. It is highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T and B lymphocytes and NK cells. Siglec-9 plays a role in negative regulation of T cell activation, and it also affects neutrophil apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-9 fused to Fc region of human IgG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA, ICC, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence staining2, Western blotting2, immunoprecipitation2, and ELISA3.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhang J, et al. 2000. J. Biol. Chem. 275:22121.
  2. Avril T, et al. 2004. J. Immunol. 173:6841. (IF, IP, WB)
  3. Biedermann B, et al. 2007. Leukemia Res. 31:221. (ELISA)
Product Citations
  1. Arunachalam PS, et al. 2021. Nature. 596:410. PubMed
RRID
AB_10896430 (BioLegend Cat. No. 351502)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T cells and B lymphocytes and NK cells

Ligand/Receptor
Sialic acid
Cell Type
Monocytes, Neutrophils
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Ikehara Y, et. al. 2004. J. Biol. Chem. 279:43117.
2. von Gunten S, et al. 2005. Blood 106:1423.

Gene ID
27180 View all products for this Gene ID
UniProt
View information about Siglec-9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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