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PE anti-human CD133 Antibody

Pricing & Availability
Clone
W6B3C1 (See other available formats)
Regulatory Status
RUO
Other Names
Prominin-1, PROM1, AC133
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W6B3C1_PE_CD133_Antibody_050824
Human peripheral blood mononuclear cells were stained with CD34 FITC and CD133 (Prominin-1) (clone W6B3C1) PE (left) or mouse IgG1, κ PE isotype control (right). Data shown was gated on the CD14 negative lymphocytes population.
  • W6B3C1_PE_CD133_Antibody_050824
    Human peripheral blood mononuclear cells were stained with CD34 FITC and CD133 (Prominin-1) (clone W6B3C1) PE (left) or mouse IgG1, κ PE isotype control (right). Data shown was gated on the CD14 negative lymphocytes population.
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397903 25 tests £105
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397904 100 tests £255
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Description

CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WERI-RB-1 human retinoblastoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

W6B3C1 antibody does not compete against AC133, 293C2, clone 7, S16015F, S16016B, or S16016E antibody binding to target.

Application References

(PubMed link indicates BioLegend citation)
  1. Hermansen SK, et al. 2011. J. Histochem Cytochem. 59(4): 391.
  2. Rappa G, et al. 2008. Stem Cells. 26(12): 3008.
  3. Tardito S, et al. 2015. Nat Cell Biol. 17(12): 1556.
RRID
AB_2860990 (BioLegend Cat. No. 397903)
AB_2860990 (BioLegend Cat. No. 397904)

Antigen Details

Structure
120 kD pentaspan transmembrance glycoprotein with 5 transmembrane domains.
Distribution

Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells.

Function
May play a role in cell differentiation, proliferation, and apoptosis.
Interaction
NAT8; NAT8B; CDHR1
Cell Type
Hematopoietic stem and progenitors, Leukemia, Leukocytes
Biology Area
Cancer Biomarkers, Cell Biology, Immuno-Oncology
Molecular Family
CD Molecules
Antigen References
  1. Yin AH, et al. 1997. Blood. 90:5002.
  2. Miraglia S, et al. 1997. Blood. 90:5013.
  3. Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25.
Gene ID
8842 View all products for this Gene ID
UniProt
View information about CD133 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 07/17/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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