PE anti-human CD58 LFA-3 Antibody anti-CD58

Pricing & Availability

Clone: TS2/9 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte function-associated antigen 3, LFA-3
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes stained with TS2/9 PE

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330905

25 tests

£84

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330928

100 tests

£226

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Description

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3) is a 45-70 kD cell surface protein that is a member of the immunoglobulin superfamily. Alternative splicing of CD58 gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored forms on cell surface. CD58 is expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells. CD58 expressed on antigen presenting cells and target cells enhances T cell recognition via the binding of it's cognate ligand, CD2, on the T cell surface.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human cytolytic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications include: immunoprecipitation¹, inhibition of cytolytic activity¹, augment of IL-1 release by TE cells²
RRID: AB_1186063 (BioLegend Cat. No. 330905)
AB_1186063 (BioLegend Cat. No. 330928)

Antigen Details

Structure: Immunoglobulin superfamily member, 45-70 kD cell surface protein. Alternative splicing gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored form on cell surface.
Distribution: Expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells.
Function: Mediates adhesion between various cell types (target cells and cytotoxic cells, APC and T cells, thymocytes and thymic epithelia).
Ligand/Receptor: CD2
Cell Type: B cells, Dendritic cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Springer TA, et al. 1987. Annu. Rev. Immunol. 5:223.
2. Dustin ML, et al. 1987. Nature 329:846.
3. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861.
4. Sanders ME, et al. 1988. J. Immunol. 140:1401.
Gene ID: 965 View all products for this Gene ID
UniProt: View information about CD58 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD58 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD58 (LFA-3)	TS2/9	FC,IP
PE anti-human CD58 (LFA-3)	TS2/9	FC
PE/Cyanine5 anti-human CD58	TS2/9	FC
PerCP/Cyanine5.5 anti-human CD58 (LFA-3)	TS2/9	FC
PE/Cyanine7 anti-human CD58 (LFA-3)	TS2/9	FC
APC anti-human CD58 (LFA-3)	TS2/9	FC
TotalSeq™-A0174 anti-human CD58 (LFA-3)	TS2/9	PG
TotalSeq™-C0174 anti-human CD58 (LFA-3)	TS2/9	PG
Ultra-LEAF™ Purified anti-human CD58 (LFA-3)	TS2/9	FC,IP,IHC,Block
TotalSeq™-B0174 anti-human CD58 (LFA-3)	TS2/9	PG
TotalSeq™-D0174 anti-human CD58 (LFA-3)	TS2/9	PG
PE anti-human CD58	TS2/9	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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