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PE anti-human CD72 Antibody

Pricing & Availability
Clone
3F3 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD72.1
Other Names
Lyb-2, Ly-19.2, Ly-32.2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
3F3_PE_CD72_Antibody_102419.png
Human peripheral blood lymphocytes were stained with CD19 FITC and anti-human CD72 (clone 3F3) PE (left) or Mouse IgG2b, κ PE isotype control (right).
  • 3F3_PE_CD72_Antibody_102419.png
    Human peripheral blood lymphocytes were stained with CD19 FITC and anti-human CD72 (clone 3F3) PE (left) or Mouse IgG2b, κ PE isotype control (right).
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316207 25 tests £84
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316208 100 tests £263
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Description

CD72 is a 39-43 kD type II membrane glycoprotein. It is a disulfide-linked homodimer belonging to C-type lectin family. CD72 is a pan-B cell marker expressed on pre-pre-B cells throughout B cell differentiation with the exception of plasma cells. It is also expressed on follicular dendritic cells, splenic red pulp macrophages (but not on peripheral blood monocytes), and liver Kupffer cells. CD72, a negative coreceptor of B cells, contains immunoreceptor tyrosine-based inhibitory motifs in the cytoplasmic domain which has been shown to recruit the tyrosine phosphatase SHP-1. Ligation of CD72 with its ligand regulates CD72 tyrosine dephosphorylation and SHP-1 dissociation to promote B cell activation and proliferation. CD100 and CD5 have been shown to be CD72 ligands. The CD100-CD72 interaction plays a role in maintenance of B cell homeostasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Product Citations
  1. Chappert P, et al. 2022. Immunity. 55:1872. PubMed
  2. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
  3. Zhao M, et al. 2021. Ann Transl Med. 9:1476. PubMed
RRID
AB_2819946 (BioLegend Cat. No. 316207)
AB_2819946 (BioLegend Cat. No. 316208)

Antigen Details

Structure
Type II membrane glycoprotein, C-type lectin family, 39-43 kD, homodimer
Distribution

Pre-pre-B cells throughout B cells differentiation (but not on plasma cells), follicular dendritic cells, splenic red pulp macrophages, liver Kupffer cells

Function
negative regulation of B cell receptor signaling
Ligand/Receptor
CD5, CD100/Sema4D
Cell Type
B cells, Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schwarting T, et al. 1992. Am. J. Hematol. 41:151.
  3. Wu HJ and S. Bondata. 2002. Immunol. Res. 25:155.
  4. Kumanogoh A, et al. 2000. Immunity 13:621.
  5. Parnes JR and C. Pan. 2000. Immunol. Rev. 176:75.
  6. Kumanogoh A, et al. 2005. Intnl. Immunol. 17:1277.
Gene ID
971 View all products for this Gene ID
UniProt
View information about CD72 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 10/23/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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