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PE anti-human CD32B/C Antibody

Pricing & Availability
Clone
4F5/CD32 (See other available formats)
Regulatory Status
RUO
Other Names
FCGR2B, FCgRIIB, FCGR2C, FCRIIC, IGFR2, CD32
Isotype
Mouse IgG1, κ
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Product Citations
publications
4F5slashCD32_PE_CD32BslashC_Antibody_020822
Human peripheral blood mononuclear cells were stained with anti-human CD19 FITC and anti-human CD32B/C (clone 4F5/CD32) PE (left) or mouse IgG1, κ PE isotype control (right).
  • 4F5slashCD32_PE_CD32BslashC_Antibody_020822
    Human peripheral blood mononuclear cells were stained with anti-human CD19 FITC and anti-human CD32B/C (clone 4F5/CD32) PE (left) or mouse IgG1, κ PE isotype control (right).
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398403 25 tests £126
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398404 100 tests £230
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Description

CD32B (FCGR2B) and CD32C (FCGR2C) are 40 kDa, type I transmembrane proteins that are members of the Ig superfamily of low-affinity immunoglobulin gamma Fc receptors. CD32B has a cytoplasmic tail that contains an immunoreceptor tyrosine-based inhibition motif (ITIM), while CD32C contains an immunoreceptor tyrosine-based activation motif (ITAM). CD32B and CD32C are low affinity receptor for monomeric IgG but also bind IgG complexes. CD32B and CD32C are expressed on B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells, and is a negative regulator of cell activation, proliferation, endocytosis, phagocytosis, and degranulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of human CD32B
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

As the extracellular region of CD32B and CD32C are identical, this Ab recognizes both isoforms. Does not crossreact with CD32A.

Application References

(PubMed link indicates BioLegend citation)
  1. Su K, et al. 2007. J Immunol. 178:3272
RRID
AB_2910458 (BioLegend Cat. No. 398403)
AB_2910458 (BioLegend Cat. No. 398404)

Antigen Details

Structure
Type I transmembrane protein, 40 kDa, member of the Ig superfamily
Distribution

B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells.

Function
Negatively regulates cell activation, proliferation, endocytosis, phagocytosis, and degranulation
Ligand/Receptor
Fc region of complex or aggregated immunoglobulins gamma
Cell Type
B cells, Dendritic cells, Granulocytes, Monocytes, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Bruhns P, et al. 2009. Blood. 113: 3716.
  2. Bewarder N, et al. 1996. Mol Cell Biol. 16: 4735.
  3. Descours B, et al. 2017. Nature 543: 564.
  4. Tomiyama Y, et al. 1992. Blood. 80: 2261.
  5. Indik Z, et al. 1991. J Clin Invest. 88: 1766.
  6. Ramsland PA, et al. 2011. J Immunol. 187: 3208.
  7. Hogarth PM and Pietersz GA. 2012. Nat Rev Drug Discov. 11: 311.
  8. Bournazos S, et al. 2009. J Immunol. 182: 8026.
  9. Maxwell KF, et al. 1999. Nat Struct Biol. 6: 437.
  10. Sandilands GP, et al. 1997. Immunology. 91: 204.
  11. Ghazizadeh S, et al. 1994. J Biol Chem. 269: 8878.
  12. Gillis C, et al. 2014. Front Immunol. 5: 254.
Gene ID
2213 View all products for this Gene ID 9103 View all products for this Gene ID
UniProt
View information about CD32B CD32C on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/08/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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