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PE anti-human FCGR2A/CD32A Antibody

Pricing & Availability
Clone
S20004A (See other available formats)
Regulatory Status
RUO
Other Names
FcγRIIa
Isotype
Mouse IgG1, κ
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Product Citations
publications
S20004A_PE_FCGR2A_CD32A_Antibody_111721
Human peripheral blood monocytes stained with anti-human FCGR2A/CD32A (clone S20004A) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • S20004A_PE_FCGR2A_CD32A_Antibody_111721
    Human peripheral blood monocytes stained with anti-human FCGR2A/CD32A (clone S20004A) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
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365503 25 tests £81
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365504 100 tests £169
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Description

CD32A (FCGR2A) is a 40 kD, type I protein with 2 C2-set domains the extracellular region and 1 ITAM in the intracellular region. CD32A binds monomeric IgG with low affinity but is very efficient binding immune complexes.  FCGR2A is expressed by myeloid cells such as macrophages, monocytes, neutrophils, eosinophils and dendritic cells; CD32a is also expressed by epithelial cells, platelets and activated CD4+ T cells. CD32a is involved in phagocytosis and clearing of immune complexes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
FCGR2A-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

 This clone does not recognizes CD32B-transfected cell

RRID
AB_2904394 (BioLegend Cat. No. 365503)
AB_2904394 (BioLegend Cat. No. 365504)

Antigen Details

Structure
Member of the Ig superfamily, 40 kD, type I protein, 2 C2-set domains, 1 ITAM
Distribution

Macrophages, monocytes, neutrophils, eosinophil, platelets, and CD4+ T Cells

Function
Phagocytosis, cytotoxicity, platelet aggregation
Ligand/Receptor
Aggregated IgG, immune complexes
Cell Type
Eosinophils, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets
Biology Area
Adaptive Immunity, Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Hubbard J. et al. 2020. J Exp Med. 217:e20200359
  2. Newling M, et al. 2020. Clin Exp Immunol. 199:39-49
  3. Maria H, et al. 2018. Front Immunol. 9:2814
  4. Descours B, et al. 2017. Nature. 543:564-567
Gene ID
NA
UniProt
View information about FCGR2A/CD32A on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/17/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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