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PE anti-human IL-16 Antibody

Pricing & Availability
Clone
14.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-16
Isotype
Mouse IgG2a
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Product Citations
publications
14.1_PE_1_082911
Human peripheral blood lymphocytes were surface stained with CD3 APC, and then fixed, permeabilized, and stained with IL-16 (clone 14.1) PE (top) or mouse IgG2a, κ PE (bottom).
  • 14.1_PE_1_082911
    Human peripheral blood lymphocytes were surface stained with CD3 APC, and then fixed, permeabilized, and stained with IL-16 (clone 14.1) PE (top) or mouse IgG2a, κ PE (bottom).
  • 14.1_PE_2_082911
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519106 100 tests £205
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Description

Interleukin-16 is a proinflammatory cytokine synthesized as a 68 kD precursor molecule (pro-IL-16). After cleavage of pro-IL-16 by caspase-3, IL-16 is released as a 12 kD protein, but its biologic activities are exerted by a tetramer. IL-16 is a chemoattractant for CD4+ T cells, monocytes, and eosinophils. On T cells, IL-16 upregulates CD25 and class II expression, and also inhibits HIV-1 replication in vitro by repressing the transcription of the HIV-1 long terminal repeat. IL-16 is expressed by lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts. CD4 and CD9 are the receptors of IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide corresponding to amino acids 615 to 630 (RRKSLQSKETTAAGDS) of IL-16
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA2, inhibition of T cell chemotaxis2 and immunohistochemical staining of frozen lymph node sections3.

Application References

(PubMed link indicates BioLegend citation)
  1. Cruikshank WW, et al. 1994. P. Natl Acad Sci USA 91:5109. (ICFC)
  2. Keane J, et al. 1998. J. Immunol. 160:5945. (Block, ELISA)
  3. Chupp GL, et al. 1998. J. Immunol. 161:3114. (Block, IHC)
  4. Stoitzner P, et al. 2001. J. Invest. Dermatol. 116:641. (ICFC)
  5. Ren F, et al. 2005. J. Immunol. 174:2738. (ICFC)
Product Citations
  1. Noman M, et al. 2015. Cancer Res . 75: 3771-3787. PubMed
RRID
AB_10897940 (BioLegend Cat. No. 519106)

Antigen Details

Structure
12 kD protein with a PDZ domain, product of the cleavage of pro-IL-16 by caspase-3, forms tetramers.
Distribution

Lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts.

Function
Chemoattractant for CD4+ T cells, monocytes, and eosinophils. Upregulates CD25 and class II expression on T cells. Inhibits HIV-1 replication.
Ligand/Receptor
CD4.
Cell Type
Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Macrophages, Mast cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Bellon M, et al. 2010. Blood 115:2441.
2. Rofani C, et al. 2009. Stem Cells Dev. 18:671.
3. Cruikshank W and Little F. 2008. Crit. Rev. Immunol. 28:467.
4. Gu Y, et al. 2008. J. Immunol. 181:4418.
5. McFadden C, et al. 2007. J. Immunol. 179:6439.
6. Qi JC, et al. 2006. Blood 107:135.

Gene ID
3603 View all products for this Gene ID
UniProt
View information about IL-16 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 05/22/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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