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PE anti-Lck Phospho (Tyr394) Antibody

Pricing & Availability
Clone
A18002D (See other available formats)
Regulatory Status
RUO
Other Names
Lsk, p56lck, LCK Proto-Oncogene, Src Family Tyrosine Kinase, Tyrosine-protein kinase LCK, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, T cell-specific protein-tyrosine kinase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A18002D_PE_Lck-Phospho_Antibody_110719.png
Human peripheral blood lymphocytes were treated with (left), or without (right) hydrogen peroxide for 5 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002), then surfaced stained with CD3 APC and intracellularly stained with anti-Lck Phospho (Tyr394) (clone A18002D) PE.
  • A18002D_PE_Lck-Phospho_Antibody_110719.png
    Human peripheral blood lymphocytes were treated with (left), or without (right) hydrogen peroxide for 5 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002), then surfaced stained with CD3 APC and intracellularly stained with anti-Lck Phospho (Tyr394) (clone A18002D) PE.
See PE spectral data
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933103 25 tests £100
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933104 100 tests £255
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Description

The Src family tyrosine kinase p56Lck (Lck) is a non-receptor tyrosine kinase that plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. Lck, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complexes results in the recruitment of CD4- and CD8-bound Lck to the TCR/CD3 signaling complex. Lck then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by Lck. Lck also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of Lck by CSK at tyrosine 505 negatively regulates the kinase, and is proposed to generate a closed, inactive conformation of the protein. Conversely, phosphorylation at tyrosine 394 in the activation loop of Lck greatly stimulates enzymatic activity. In many types of cancer, Lck is a proliferative and anti-apoptotic driver, and has been proposed as a target for therapeutic intervention.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human Src phosphorylated at tyrosine 419
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone has not been tested for IP.

This clone may recognize other Src family members.

This clone was ICC tested on PFA-fixed cells using both methanol and Triton X-100 permeabilization methods. Both permeabilazation methods were compatible with staining. Triton X-100 permeabilization produces stronger staining.

During ICC product development testing, this clone produced strong nuclear staining in a small subpopulation (<1%) of both unstimulated and stimulated cells. The source of this staining is not clear but we do not believe it is Lck-dependent.

This clone was developed using a synthetic peptide corresponding to human Src phosphorylated at tyrosine 419, which displays high sequence homology with the region surrounding Lck tyrosine 394. To confirm specificity to Lck Phospho (Tyr394), the cell line J.Cam1.6 was used. This cell line is a clonal derivative of Jurkat cells that harbors a truncated LCK allele with defective protein expression (Straus, et al. 1992. Cell. 70:585).

Product Citations
  1. Reed JC, et al. 2020. PLoS One. 15:e0236921. PubMed
RRID
AB_2820203 (BioLegend Cat. No. 933103)
AB_2820203 (BioLegend Cat. No. 933104)

Antigen Details

Structure
Lck is a 509 amino acid protein with a predicted molecular weight of 58 kD
Distribution

Plasma-membrane associated/T cells

Function
Protein Tyrosine Kinase
Cell Type
T cells
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Phillipsen L, et al. 2017. Sci Signal. 10:eaaf4736.
  2. Moogk D, et al. 2016. J Immunol. 197:644.
  3. Klammt C, et al. 2015. Nat Immunol. 16:961.
  4. Cancer Genome Atlas Network. 2015. Cell. 161:1681.
  5. Casas J, et al. 2014. Nat Commun. 5:5624
  6. McNeill L, et al. 2007. Immunnity. 27:425.
  7. Lefebvre DC, et al. 2003. Biochim Biophys Acta. 1650:40.
  8. Kabouridis PS. et al. 2003. Biochem J. 371:907.
  9. Abraham N, et al. 1990. Mol. Cell. Biol. 10:5197.
Gene ID
3932 View all products for this Gene ID
UniProt
View information about Lck Phospho Tyr394 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/07/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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