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PE anti-Lck Phospho (Tyr505) Antibody

Pricing & Availability
Clone
A17013A (See other available formats)
Regulatory Status
RUO
Other Names
Leukocyte C-terminal Src kinase, Protein YT16, p56-Lck, T cell-specific protein-tyrosine kinase
Isotype
Mouse IgG1, κ
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Product Citations
publications
A17013A_PE_LCK-Phospho-Tyr505_Antibody_123118
Human peripheral blood lymphocytes were left untreated (left) or treated with Hydrogen Peroxide for 5 minutes (right), fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then surfaced stained with CD3 APC and intracellularly stained with anti-LCK Phospho (Tyr505) (clone A17013A) PE.
  • A17013A_PE_LCK-Phospho-Tyr505_Antibody_123118
    Human peripheral blood lymphocytes were left untreated (left) or treated with Hydrogen Peroxide for 5 minutes (right), fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then surfaced stained with CD3 APC and intracellularly stained with anti-LCK Phospho (Tyr505) (clone A17013A) PE.
See PE spectral data
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699703 25 tests £100
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699704 100 tests £255
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Description

The Src family tyrosine kinase p56Lck (Lck) is a non-receptor tyrosine kinase that is primarily expressed in T cells and NK cells. It plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. Lck, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complex results in the recruitment of CD4- and CD8-bound Lck to the TCR/CD3 signaling complex. Lck then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by Lck. Lck also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of Lck by CSK at Tyrosine 505 negatively regulates Lck, and is proposed to generate a closed, inactive conformation of the protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Lck peptide phosphorylated at Tyr 505.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2801136 (BioLegend Cat. No. 699703)
AB_2801136 (BioLegend Cat. No. 699704)

Antigen Details

Structure
509 and 539 amino acid isoforms with predicted molecular weights of 58 and 61 kD, respectively. Contains N-terminal domain with myristylation and palmitoylation sites, a kinase domain, and SH2 and SH3 domains that mediate protein-protein interactions between Lck and other proteins.
Distribution

T cells

Function
T cell receptor signaling
Interaction
AXL, CD2, CD4, CD5, CD8, CD44, CD45, CD122, PI4K, VAV1, RASA1, FYB, CDK1, RAF1, ZAP70, SYK, RUNX3
Cell Type
T cells
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Phillipsen L, et al. 2017. Sci Signal. 10:eaaf4736.
  2. Moogk D, et al. 2016. J Immunol. 197:644.
  3. Klammt C, et al. 2015. Nat Immunol. 16:961.
  4. Cancer Genome Atlas Network. 2015. Cell. 161:1681.
  5. Casas J, et al. 2014. Nat Commun. 5:5624
  6. McNeill L, et al. 2007. Immunnity. 27:425.
  7. Lefebvre DC, et al. 2003. Biochim Biophys Acta. 1650:40.
  8. Kabouridis PS. 2003. Biochem J. 371:907.
Gene ID
3932 View all products for this Gene ID
UniProt
View information about Lck Phospho Tyr505 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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