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FITC anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
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Product Citations
publications
N418_A_FITC_Pos_071006
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
  • N418_A_FITC_Pos_071006
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
  • N418_B_FITC_Neg_071006
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
Compare all formats See FITC spectral data
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117305 50 µg £37
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117306 500 µg £80
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Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  2. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  3. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  4. Man SM et al. 2016. Cell. 167(2):382-396 . PubMed
  5. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  6. Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed
  7. Zhao J, et al. 2021. Emerg Microbes Infect. 10:913. PubMed
  8. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  9. Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed
  10. Corral D, et al. 2022. Cell Rep. 39:110715. PubMed
  11. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  12. Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed
  13. Yu C, et al. 2022. Invest Ophthalmol Vis Sci. 63:1. PubMed
  14. Minichino D, et al. 2022. J Leukoc Biol. 112:1089. PubMed
  15. Sun Y, et al. 2023. Bioact Mater. 22:47. PubMed
  16. Zhang Z, et al. 2022. Integr Cancer Ther. 21:15347354221138534. PubMed
  17. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  18. Yu J, et al. 2022. Nat Commun. 13:7903. PubMed
  19. Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed
  20. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  21. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
  22. Liu H, et al. 2023. Cell Death Differ. :. PubMed
  23. Li X, et al. 2022. Cell. 185:1709. PubMed
  24. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  25. Akhtar MN, et al. 2022. MBio. 13:e0330921. PubMed
  26. Li Q, et al. 2022. Biomed Pharmacother. 153:113459. PubMed
  27. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  28. Chen H, et al. 2023. Nat Commun. 14:941. PubMed
  29. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  30. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  31. Cheng K, et al. 2023. Small. 19:e2300125. PubMed
  32. Sang H, et al. 2023. Adv Clin Exp Med. 32:551. PubMed
  33. Huang T, et al. 2023. Front Immunol. 14:1124152. PubMed
  34. Fu S, et al. 2023. Nat Commun. 14:2248. PubMed
  35. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  36. Li T, et al. 2023. Nat Commun. 14:2498. PubMed
  37. Wu N, et al. 2023. Front Bioeng Biotechnol. 11:1173381. PubMed
  38. Wang K, et al. 2023. Nat Commun. 14:2950. PubMed
  39. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  40. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  41. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  42. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  43. Feola S, et al. 2022. Front Immunol. 13:826164. PubMed
  44. Wilhelm J, et al. 2021. J Control Release. 329:353. PubMed
  45. Larson-Casey JL, et al. 2021. FASEB J. 35:e21675. PubMed
  46. Chen Y, et al. 2018. EBioMedicine. 32:31. PubMed
  47. Zhu H, et al. 2018. Elife. 7:. PubMed
  48. Devenport SN, et al. 2021. JCI Insight. 6: . PubMed
  49. Jiang W, et al. 2021. Oncol Lett. 22:625. PubMed
  50. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  51. Chen X, et al. 2021. Theranostics. 11:4655. PubMed
  52. Zhang S, et al. 2022. Acta Pharm Sin B. 12:3124. PubMed
  53. Xing J, et al. 2016. Nat Immunol. 17:1373-1380. PubMed
  54. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  55. Pott J et al. 2018. Cell host & microbe. 23(2):191-202 . PubMed
  56. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  57. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  58. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  59. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  60. Wang X, et al. 2021. Front Cell Dev Biol. 632805:9. PubMed
  61. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  62. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  63. Schwartz AJ, et al. 2021. Nat Metab. 3:969. PubMed
  64. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  65. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  66. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  67. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  68. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  69. Wu J, et al. 2021. Mol Med Rep. 23:. PubMed
  70. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  71. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  72. Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
  73. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  74. Shin JE, et al. 2021. Heliyon. 7:e08433. PubMed
  75. Jin R, et al. 2020. Mol Cancer Ther. . PubMed
  76. Marjanovic ND, et al. 2020. Cancer Cell. 38(2):229-246.e13. PubMed
  77. Ippolito G, et al. 2014. Proc Natl Acad Sci U S A. 111:998. PubMed
  78. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  79. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  80. Xiao Y, et al. 2022. Clin Transl Med. 12:e1071. PubMed
  81. Shen J, et al. 2022. Mater Today Bio. 16:100416. PubMed
  82. Zhang L, et al. 2021. J Immunother Cancer. 9:. PubMed
  83. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  84. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  85. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  86. El-Zaatari M, et al. 2014. J Immunol. 193:807. PubMed
  87. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  88. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  89. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  90. Coe D, et al. 2022. JCI Insight. 7:. PubMed
  91. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  92. Bharali D, et al. 2008. Nanomedicine. 4:311. PubMed
  93. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  94. Wan X, et al. 2018. Nature. 560:107. PubMed
  95. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  96. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  97. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  98. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  99. Xue RY, et al. 2019. Front Immunol. 10:1372. PubMed
  100. Fu Z, et al. 2021. Cell Res. 31:631. PubMed
  101. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
  102. Mao L, et al. 2022. Front Immunol. 13:833418. PubMed
  103. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  104. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  105. Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
  106. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  107. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  108. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  109. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  110. Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed
  111. Patankar Y, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L902 - L913. PubMed
  112. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
  113. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  114. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  115. Han W, et al. 2020. J Immunol. 205:1125. PubMed
  116. Feng Y, et al. 2022. J Nanobiotechnology. 20:193. PubMed
  117. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  118. Nabet BY et al. 2017. Cell. 170(2):352-366 . PubMed
  119. Bi E, et al. 2018. J Clin Invest. 128:4821. PubMed
  120. Li Z, et al. 2022. Nat Commun. 13:6321. PubMed
  121. Hsu M, et al. 2022. Nat Immunol. 23:581. PubMed
  122. Miki Y, et al. 2022. JCI Insight. 7:. PubMed
  123. Yi H, et al. 2021. Front Immunol. 12:719189. PubMed
  124. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  125. Jaiswal M, et al. 2014. J Leukoc Biol. 96:337. PubMed
  126. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  127. Resch TK, et al. 2018. Sci Rep. 0.722916667. PubMed
  128. Chin R, et al. 2006. J Immunol. 177:290. PubMed
  129. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  130. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  131. Zhang J, et al. 2022. Biomater Res. 26:44. PubMed
  132. Nasser H, et al. 2020. Cell Death Discov. 0.29375. PubMed
  133. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  134. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  135. Qin S, et al. 2018. Oncol Lett. 128:1283. PubMed
  136. Gu H, et al. 2021. Elife. 10:. PubMed
  137. Yang Z, et al. 2021. Nat Commun. 12:4299. PubMed
  138. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  139. Poncini C, et al. 2015. J Immunol. 195: 3311 - 3324. PubMed
  140. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  141. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  142. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  143. Barrera-Avalos C, et al. 2021. iScience. 24:103520. PubMed
  144. Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed
  145. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  146. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  147. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  148. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  149. Li Z, et al. 2022. Evid Based Complement Alternat Med. 2022:7421265. PubMed
  150. Wang X, et al. 2022. Elife. 11:. PubMed
  151. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  152. Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed
  153. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  154. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  155. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  156. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  157. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  158. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  159. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  160. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  161. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  162. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  163. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  164. Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed
  165. Cao Y, et al. 2022. Small. 18:e2203466. PubMed
  166. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  167. Lin YC, et al. 2020. Mol Med Rep. 22:4909. PubMed
  168. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
RRID
AB_313774 (BioLegend Cat. No. 117305)
AB_313774 (BioLegend Cat. No. 117306)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Other Formats

View All CD11c Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD11c N418 FC
Biotin anti-mouse CD11c N418 FC,IHC-F
FITC anti-mouse CD11c N418 FC
PE anti-mouse CD11c N418 FC
Purified anti-mouse CD11c N418 FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c N418 FC
Alexa Fluor® 647 anti-mouse CD11c N418 FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c N418 FC
PE/Cyanine7 anti-mouse CD11c N418 FC
Brilliant Violet 605™ anti-mouse CD11c N418 FC
Alexa Fluor® 700 anti-mouse CD11c N418 FC
Pacific Blue™ anti-mouse CD11c N418 FC
APC/Cyanine7 anti-mouse CD11c N418 FC
PerCP/Cyanine5.5 anti-mouse CD11c N418 FC
PerCP anti-mouse CD11c N418 FC
Brilliant Violet 421™ anti-mouse CD11c N418 FC,IHC
Brilliant Violet 570™ anti-mouse CD11c N418 FC
Brilliant Violet 785™ anti-mouse CD11c N418 FC
Brilliant Violet 510™ anti-mouse CD11c N418 FC
Brilliant Violet 650™ anti-mouse CD11c N418 FC
Purified anti-mouse CD11c (Maxpar® Ready) N418 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c N418 IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c N418 FC
Brilliant Violet 711™ anti-mouse CD11c N418 FC
APC/Fire™ 750 anti-mouse CD11c N418 FC
TotalSeq™-A0106 anti-mouse CD11c N418 PG
Brilliant Violet 750™ anti-mouse CD11c N418 FC
TotalSeq™-B0106 anti-mouse CD11c N418 PG
TotalSeq™-C0106 anti-mouse CD11c N418 PG
KIRAVIA Blue 520™ anti-mouse CD11c N418 FC
Spark Blue™ 550 anti-mouse CD11c N418 FC
Spark NIR™ 685 anti-mouse CD11c N418 FC
Spark UV™ 387 anti-mouse CD11c N418 FC
Spark Red™ 718 anti-mouse CD11c N418 FC
Spark Blue™ 515 anti-mouse CD11c N418 FC
PerCP/Fire™ 806 anti-mouse CD11c N418 FC
Spark PLUS UV395™ anti-mouse CD11c N418 FC
Spark Blue™ 574 anti-mouse CD11c (Flexi-Fluor™) N418 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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