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PE anti-mouse CD371 (CLEC12A) Antibody

Pricing & Availability
Clone
5D3/CLEC12A (See other available formats)
Regulatory Status
RUO
Other Names
CLL1, MICL, CLL-1, DCAL-2, C-type lectin domain family 12 member A, CD371
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5D3slashCLEC12A_1_020812
C57BL/6 mouse splenocytes were stained with CD8 PerCP, CD11c APC, and CD371 (CLEC12A, clone 5D3/CLEC12A) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data was gated on CD8 positive cell population.
  • 5D3slashCLEC12A_1_020812
    C57BL/6 mouse splenocytes were stained with CD8 PerCP, CD11c APC, and CD371 (CLEC12A, clone 5D3/CLEC12A) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data was gated on CD8 positive cell population.
  • 5D3slashCLEC12A_2_020812
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143403 25 µg £81
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143404 100 µg £230
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Description

CD371 (CLEC12A), also known as DCAL-2, MICL or CLL-1, is a 30 kD type II transmembrane protein with extracellular C-type lectin domains, belonging to the C-type lectin family.  Its cytoplasmic ITIM motif modulates signaling cascades and is involved in phosphorylation of tyrosine residues in MAP kinases. Mouse CLEC12A is expressed on CD8+ dendritic cells (DCs), a proportion of CD8- dendritc cells, plasmacytoid DCs, monoctyes, macrophages, granulocytes, and B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO expressing mouse CLEC12A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Raulf MK, et al. 2019. Cell Rep. 28:30. PubMed
  2. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  3. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
RRID
AB_11126747 (BioLegend Cat. No. 143403)
AB_11126747 (BioLegend Cat. No. 143404)

Antigen Details

Structure
Type II C-type lectin family member, 265 aa, 30.7 kD
Distribution

CD8+ dendritic cells (DCs), a proportion of CD8- dendritic cells, plasmacytoid DCs, monocytes, macrophages, granulocytes, B cells

Function
Negative regulator of granuocyte and monocyte function
Interaction
PTPN6 and PTPN11
Cell Type
B cells, Dendritic cells, Embryonic Stem Cells, Granulocytes, Macrophages, Monocytes
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Lahoud MH, et al. 2009. J. Immunol. 182:7587.
2. Chen CH, et al. 2006. Blood 107:1459.
3. Marshall AS, et al. 2004. J. Biol. Chem. 279:14792.

Gene ID
232413 View all products for this Gene ID
UniProt
View information about CD371 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 01/15/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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