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PE/Cyanine7 anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5114_PECy7_092409
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE/Cyanine7 (orange line) or rat IgG2b, κ PE/Cyanine7 isotype control (purple line).
  • M5114_PECy7_092409
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE/Cyanine7 (orange line) or rat IgG2b, κ PE/Cyanine7 isotype control (purple line).
Compare all formats See PE/Cyanine7 spectral data
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107629 25 µg £65
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107630 100 µg £200
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  2. Shi M, et al. 2021. Theranostics. 11:2349. PubMed
  3. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  4. Zhao J, et al. 2021. Emerg Microbes Infect. 10:913. PubMed
  5. Motyka NI, et al. 2022. Infect Immun. 90:e0063721. PubMed
  6. Fan R, et al. 2023. J Immunother Cancer. 11: . PubMed
  7. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
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  11. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  12. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  13. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  14. Pandey S, et al. 2020. Cell Syst. 11:495. PubMed
  15. Rosa FF, et al. 2020. Bio Protoc. 10:e3619. PubMed
  16. Pingili AK, et al. 2021. Cell Reports. 35(12):109285. PubMed
  17. Grohmann M et al. 2018. Cell. 175(5):1289-1306 . PubMed
  18. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  19. Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed
  20. Zhang J, et al. 2021. J Leukoc Biol. 110:485. PubMed
  21. Rolfes S, et al. 2020. Neurobiol Dis. 144:105024. PubMed
  22. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  23. Sheng K, et al. 2014. PLoS One. 9:95208. PubMed
  24. Wei SC, et al. 2020. Cancer Discov. . PubMed
  25. Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
  26. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  27. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  28. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  29. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
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  34. Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed
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  41. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
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  45. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  46. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  47. He C, et al. 2022. Nat Commun. 13:5459. PubMed
  48. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  49. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  50. Kriegsman BA, et al. 2019. J Immunol. 203:1999. PubMed
  51. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  52. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  53. Wei Y, et al. 2021. Endocrinology. 162(8):. PubMed
  54. Natale CA, et al. 2018. Elife. 7. PubMed
  55. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  56. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  57. Chen B, et al. 2021. Cell. 184:6262. PubMed
  58. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  59. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  60. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  61. Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
  62. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
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RRID
AB_2069376 (BioLegend Cat. No. 107629)
AB_2069376 (BioLegend Cat. No. 107630)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10/30/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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