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PE anti-mouse Ly-51 Antibody

Pricing & Availability
Clone
6C3 (See other available formats)
Regulatory Status
RUO
Other Names
6C3/BP-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
6c3
C57BL/6 mouse bone marrow cells stained with 6C3 PE and B220 FITC
  • 6c3
    C57BL/6 mouse bone marrow cells stained with 6C3 PE and B220 FITC
Compare all formats See PE spectral data
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108307 50 µg £81
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108308 200 µg £221
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Description

Ly-51 is a 140 kD protein also known as 6C3/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP)
  2. Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP)
  3. Adkins B, et al. 1988. Immunogenetics 27:180. (IHC)
  4. Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC)
  5. Goverman J, et al. 1997. Immunol. Today 18:204. (IHC)
  6. Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed
  7. Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC)
  8. Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)
Product Citations
  1. Padonou F, et al. 2022. EMBO Rep. 23:e53576. PubMed
  2. Fujimori S, et al. 2022. Elife. 11:. PubMed
  3. Li C, et al. 2018. Nat Commun. 1.073611111. PubMed
  4. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  5. Giraud M, et al. 2014. Proc Natl Acad Sci U S A. 111:1491. PubMed
  6. Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed
  7. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  8. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  9. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  10. Wells KL, et al. 2020. Elife. 9:. PubMed
  11. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  12. Guyon C, et al. 2020. eLife. 9:e52985.. PubMed
  13. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  14. Li J, et al. 2020. Development. :. PubMed
  15. Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed
  16. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  17. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  18. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  19. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  20. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
RRID
AB_313365 (BioLegend Cat. No. 108307)
AB_313365 (BioLegend Cat. No. 108308)

Antigen Details

Structure
Aminopeptidase A, 140 kD
Distribution

B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells

Function
Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors
Cell Type
B cells, Dendritic cells, Epithelial cells
Biology Area
Immunology
Antigen References

1. Goverman J, et al. 1997. Immunol. Today 18:204.
2. Morse HC, et al. 1987. J. Exp. Med. 165:920.
3. Sherwood P, et al. 1990. Int. Immunol. 2:399.
4. Lin Q, et al. 1998. J. Immunol. 160:4681.

Gene ID
13809 View all products for this Gene ID
UniProt
View information about Ly-51 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10/10/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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