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PE anti-mouse Ly49D Antibody

Pricing & Availability
Clone
4E5 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-49D
Isotype
Rat IgG2a, κ
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Product Citations
publications
4E5_PE_1_091010
C57BL/6 splenocytes stained with NK1.1 (PK136) Alexa Fluor® 647 and 4E5 PE (top) or rat IgG2a PE isotype control (bottom)
  • 4E5_PE_1_091010
    C57BL/6 splenocytes stained with NK1.1 (PK136) Alexa Fluor® 647 and 4E5 PE (top) or rat IgG2a PE isotype control (bottom)
  • 4E5_PE_2_091010
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138307 25 µg £72
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138308 100 µg £200
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Description

Ly49D is a type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.  Ly49D is expressed on subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL mouse strains, but not Balb/c, DBA/2, AKR and CBA/J.  The receptors form homodimers that bind to MHC class I alloantigens.  Unlike inhibitory Ly49 family members, Ly49D lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) but contains a cytoplasmic immunoreceptor tyrosine-based activation motif.  The activating receptor Ly49D delivers stimulatory signals for target cell lysis upon interacting with H2-Dd, Dr and Dsp2, but not with H2b or H2k class I antigens.  Cross-linking of Ly49D activates intracellular kinase activity and calcium mobilization in NK cells.  In 129/J mice, 4E5 antibody cross-reacts with Ly49O, LY49R and Ly49V. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking2, 3 of CHO cell or Con A-activated lymphoblast killing by NK cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Mason LH, et al. 1998. J. Immunol. 160:4148. (IP)
  2. Idris A, et al. 1999. Proc. Natl. Acad. Sci. 96:6330. (FC Block)
  3. George TC, et al. 1999. J. Immunol. 162:2035. (FC Block)
  4. Ortaldo JR, et al. 1999. J. Leukoc. Biol. 66:512. (FC)
  5. Ortaldo JR, et al. 1998. J. Immunol. 160:1158. (FC)
Product Citations
  1. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  2. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  3. Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
  4. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
RRID
AB_10639939 (BioLegend Cat. No. 138307)
AB_10639939 (BioLegend Cat. No. 138308)

Antigen Details

Structure
A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.
Distribution

Subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL, but not Balb/c, DBA/2, AKR and CBA/J.

Function
Plays an important role in activating natural killer (NK) cell cytolytic activity.
Ligand/Receptor
MHC class I alloantigens
Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613.
2. Takei F, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.

Gene ID
16635 View all products for this Gene ID
UniProt
View information about Ly49D on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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