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PE anti-rat CD25 Antibody

Pricing & Availability
Clone
OX-39 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2 receptor chain α, IL-2Rα, low affinity IL-2 receptor chain, Tac, p55, Ly-43
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-39_PE_060308
ConA-stimulated (3day) LOU splenocytes stained with OX-39 PE
  • OX-39_PE_060308
    ConA-stimulated (3day) LOU splenocytes stained with OX-39 PE
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202105 50 µg £97
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Description

CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat T cell blasts from mixed lymphocyte reactions
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1, immunoprecipitation1, weakly blocks IL-2 binding1, 2, 3, and blocks IL-2 induced epithelial cell migration3.

Application References

(PubMed link indicates BioLegend citation)
  1. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281. (IHC, IP, Block)
  2. Tellides G, et al. 1987. Transplant Proc. 19:4231. (Block)
  3. Digness AU, et al. 1996. Exp.Cell Res. 225:422. (Block)
Product Citations
  1. Xie L, et al. 2014. J Immunol. 192:6009. PubMed
  2. Yanai H, et al. 2022. Elife. 11:. PubMed
  3. Ruppert KA, et al. 2018. Sci Rep. 8:480. PubMed
  4. Zhang HC, et al. 2018. Int J Mol Med. 41:13. PubMed
  5. Abu-El-Rub E, et al. 2020. Cell Death Dis. 0.749305556. PubMed
  6. Ni X, et al. 2019. Mol Med Rep. 19:3743. PubMed
RRID
AB_314002 (BioLegend Cat. No. 202105)

Antigen Details

Structure
Type I membrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, thymic and splenic dendritic cells, intestinal epithelial cells

Function
Low affinity receptor for IL-2; associates with IL-2 receptor β (CD122) and γ (CD132) chains to form high affinity receptor
Ligand/Receptor
IL-2
Cell Type
B cells, Dendritic cells, Epithelial cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Digness AU, et al. 1996. Exp.Cell Res. 225:422.
2. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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