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PE anti-ZAP70 Antibody

Pricing & Availability
Clone
A15114B (See other available formats)
Regulatory Status
RUO
Other Names
Zeta chain associated protein kinase, SYK related tyrosine kinase, ZAP-70
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15114B_PE_ZAP70_Antibody_1_081018
Human peripheral blood lymphocytes were surface stained with CD19 Alexa Fluor® 488 then treated with the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with anti-ZAP70 (clone A15114B) PE (left) or mouse IgG2b, κ PE (right).
  • A15114B_PE_ZAP70_Antibody_1_081018
    Human peripheral blood lymphocytes were surface stained with CD19 Alexa Fluor® 488 then treated with the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with anti-ZAP70 (clone A15114B) PE (left) or mouse IgG2b, κ PE (right).
  • A15114B_PE_ZAP70_Antibody_2_081018
    Human peripheral blood lymphocytes were surface stained with CD56 APC then treated with the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with anti-ZAP70 (clone A15114B) PE (left) or mouse IgG2b, κ PE (right).
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691203 25 tests £84
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691204 100 tests £197
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Description

ZAP70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP70 have been linked to selective T cell defects. The ZAP70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-peptide surrounding ZAP70 Y319.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Reed JC, et al. 2020. PLoS One. 15:e0236921. PubMed
RRID
AB_2721648 (BioLegend Cat. No. 691203)
AB_2721648 (BioLegend Cat. No. 691204)

Antigen Details

Structure
Protein tyrosine kinase, contains two SH2 domains. Human Zap70 has three isoforms with predicted MW of 70kD, 56kD, 36kD. Mouse Zap70 has three isoforms with predicted MW of 70kD, 36kD, 35kD.
Distribution

T cells, NK cells.

Function
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP70 causes selective T cell defect.
Interaction
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated.
Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase, TCRs
Antigen References

1. Chan AC, et al. 1992. Cell. 71:649.
2. Chan AC, et al. 1994. Science. 264:1599.
3. Arpaia E, et al. 1994. Cell. 76:947.

Gene ID
7535 View all products for this Gene ID
UniProt
View information about ZAP70 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/03/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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