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PE/Cyanine5 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-PE/Cyanine5
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Product Citations
publications
SAV_PECy5_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-PE/Cyanine5
  • SAV_PECy5_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-PE/Cyanine5
See PE/Cyanine5 spectral data
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405205 100 µg £81
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-PE/Cyanine5 is useful for detecting biotinylated antibodies. The excitation of PE/Cyanine5 by 488 nm laser light induces a light emission maximum of 670 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PE/Cyanine5 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-PE/Cyanine5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular molar mass of streptavidin independent of any additional molecular mass that might be added by the PE/Cyanine5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin-Phycoerythrin/Cyanine5 (PE/Cyanine5) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Cyanine5 is 480 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Wong KA, et al. 2008. J. Immunol. 15:180. PubMed
  2. Qi Q, et al. 2009. Blood PubMed
  3. Imada M, et al. 2009. Int immunol. PubMed
  4. Sawa Y, et al. 2009. Immunity. 30:447. PubMed
  5. Song R, et al. 2010. J. Virol. PubMed
  6. Abdel-Latif A, et al. 2010. Exp Hematol. 38:1131. PubMed
  7. De Monte L, et al. 2011. J. Exp Med. 208:469. PubMed
  8. Xu B, et al. 2012. PLoS One. 7:e44719. PubMed
  9. Su ML, et al. 2014. PLoS One. 9:98823. PubMed
  10. Chen A, et al. 2014. J Immunol. 193:35. PubMed
  11. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  12. Wong EB, et al. 2015. J Immunol. 194:4130. PubMed
Product Citations
  1. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  2. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  3. Song R, et al. 2010. J Virol. 84:6935. PubMed
  4. Ogishi M, et al. 2022. J Exp Med. 219:. PubMed
  5. Abdel-Latif A, et al. 2010. Exp Hematol. 38:1131. PubMed
  6. Rodriguez K 2008. J Immunol. 180:716. PubMed
  7. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  8. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  9. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  10. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  11. Su M, et al. 2014. PLoS One. 9:98823. PubMed
  12. Imada M, et al. 2009. Int Immunol. 1.674305556. PubMed
  13. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  14. Suliman HB, et al. 2022. iScience. 25:103535. PubMed
  15. Xu B, et al. 2012. PLoS One. 7:e44719. PubMed
  16. Chen A, et al. 2014. J Immunol. 193:35. PubMed
  17. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  18. Qi Q, et al. 2009. Blood. 114:564. PubMed
  19. Pennington LF, et al. 2021. Nat Commun. 12:7069. PubMed
  20. Chiem K, et al. 2021. Microbiol Spectr. 9:e0160121. PubMed
  21. Sawa Y, et al. 2009. Immunity. 30:447. PubMed
  22. Monte L, et al. 2011. J Exp Med. 208:469. PubMed
  23. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  24. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
Go To Top Version: 4    Revision Date: 06/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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