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PE/Cyanine7 anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_PECy7_061807
Human peripheral blood lymphocytes stained with HIB19 PE/Cyanine7
  • HIB19_PECy7_061807
    Human peripheral blood lymphocytes stained with HIB19 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
302215 25 tests £98
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302216 100 tests £200
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Watanabe A, et al. 2019. Cell. 177:1124. PubMed
  2. Wu J, et al. 2022. Nat Commun. 13:676. PubMed
  3. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  4. Wildner NH, et al. 2021. J Leukoc Biol. 109:77. PubMed
  5. Liu L, et al. 2022. Sci Transl Med. 14:eabn6859. PubMed
  6. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  7. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  8. Vazquez-Lombardi R, et al. 2022. Immunity. 55:1953. PubMed
  9. Voit RA, et al. 2023. Nat Immunol. 24:69. PubMed
  10. Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed
  11. García-García A, et al. 2023. Bioact Mater. 24:174. PubMed
  12. Alkashgari HR, et al. 2022. Int J Mol Sci. 24: . PubMed
  13. Han CY, et al. 2022. Redox Biol. 51:102263. PubMed
  14. Mittler E, et al. 2022. Sci Transl Med. 14:eabl5399. PubMed
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  17. Metelo AM, et al. 2022. Cancer Res Commun. 2:158. PubMed
  18. Chernyshev M, et al. 2023. Nat Commun. 14:2249. PubMed
  19. Wu X, et al. 2023. iScience. 26:106559. PubMed
  20. Kaku CI, et al. 2023. Nat Commun. 14:2751. PubMed
  21. SoRelle ED, et al. 2022. Front Immunol. 13:1001145. PubMed
  22. Li C, et al. 2022. J Exp Med. 219:. PubMed
  23. Hubbard N, et al. 2016. Blood. 127: 2513 - 2522. PubMed
  24. Wijesinghe A, et al. 2020. J Biomed Sci. 27:50:00. PubMed
  25. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  26. Li H, et al. 2018. PLoS Genet. 14:e1007163. PubMed
  27. Hao Y, et al. 2018. Exp Ther Med. 15:1403. PubMed
  28. Browning D, et al. 2017. Gene Ther. 10.1038/gt.2016.88. PubMed
  29. Li Y, et al. 2021. Nat Commun. 12:87. PubMed
  30. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
  31. Joosten S, et al. 2016. PLoS Pathog. 12: 1005687. PubMed
  32. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  33. Cillo AR, et al. 2020. Immunity. 52(1):183-199.e9.. PubMed
  34. Amara K, et al. 2013. J Exp Med. 210:445. PubMed
  35. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  36. van Heerden D, et al. 2021. Int J Mol Sci. 22:. PubMed
  37. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  38. Masuda T, et al. 2022. iScience. 25:104781. PubMed
  39. Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed
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  42. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
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  44. Kornauth C, et al. 2021. Haematologica. 106:2251. PubMed
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  46. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  47. Szklarski M, et al. 2021. Front Immunol. 12:644548. PubMed
  48. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  49. Pellin D, et al. 2019. Nat Commun. 10:2395. PubMed
  50. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  51. Mercer F, et al. 2016. PLoS Negl Trop Dis. 10: 0004913. PubMed
  52. Menon M, et al. 2016. Immunity. 44:683-697. PubMed
  53. Lu E, et al. 2019. Nature. 567:244. PubMed
  54. Schiroli G et al. 2019. Cell stem cell. 24(4):551-565 . PubMed
  55. Roy Chowdhury R, et al. 2018. Nature. 560:644. PubMed
  56. Lindner S, et al. 2013. Cancer Res. 73:2468. PubMed
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  58. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  59. Rappazzo CG, et al. 2022. Immunity. 55:1710. PubMed
  60. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  61. Fels JM, et al. 2021. Cell. 184(13):3486-3501.e21. PubMed
  62. Zou J, et al. 2022. J Clin Invest. 132:. PubMed
  63. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  64. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  65. Crippa S, et al. 2019. J Clin Invest. 129:1566. PubMed
  66. Napolitano R, et al. 2020. Oncol Rep. 44:1561. PubMed
  67. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  68. Sakharkar M, et al. 2021. Sci Immunol. :6. PubMed
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RRID
AB_314245 (BioLegend Cat. No. 302215)
AB_314245 (BioLegend Cat. No. 302216)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD19 HIB19 FC
Biotin anti-human CD19 HIB19 FC
FITC anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Purified anti-human CD19 HIB19 FC,CyTOF®,IHC-F
APC/Cyanine7 anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
Alexa Fluor® 488 anti-human CD19 HIB19 FC,ICC
Alexa Fluor® 647 anti-human CD19 HIB19 FC,IHC-F
Pacific Blue™ anti-human CD19 HIB19 FC
Alexa Fluor® 700 anti-human CD19 HIB19 FC
PerCP anti-human CD19 HIB19 FC
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Brilliant Violet 421™ anti-human CD19 HIB19 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD19 HIB19 FC
Brilliant Violet 650™ anti-human CD19 HIB19 FC
Brilliant Violet 785™ anti-human CD19 HIB19 FC
Brilliant Violet 510™ anti-human CD19 HIB19 FC
Brilliant Violet 605™ anti-human CD19 HIB19 FC
Brilliant Violet 711™ anti-human CD19 HIB19 FC
Purified anti-human CD19 (Maxpar® Ready) HIB19 FC,CyTOF®
Alexa Fluor® 594 anti-human CD19 HIB19 ICC,IHC-F
PE/Dazzle™ 594 anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
Pacific Blue™ anti-human CD19 HIB19 FC
APC anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
TotalSeq™-A0050 anti-human CD19 HIB19 PG
Brilliant Violet 750™ anti-human CD19 HIB19 FC
TotalSeq™-B0050 anti-human CD19 HIB19 PG
TotalSeq™-C0050 anti-human CD19 HIB19 PG
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Spark NIR™ 685 anti-human CD19 HIB19 FC
Ultra-LEAF™ Purified anti-human CD19 HIB19 FC,CyTOF®,Block,IHC
APC/Fire™ 810 anti-human CD19 HIB19 FC
PE/Fire™ 640 anti-human CD19 HIB19 FC
PE/Fire™ 700 anti-human CD19 HIB19 FC
TotalSeq™-D0050 anti-human CD19 HIB19 PG
Spark YG™ 593 anti-human CD19 HIB19 FC
GMP Pacific Blue™ anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
GMP PE anti-human CD19 HIB19 FC
GMP APC anti-human CD19 HIB19 FC
KIRAVIA Blue 520™ anti-human CD19 HIB19 FC
GMP PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
GMP PE/Cyanine7 anti-human CD19 HIB19 FC
Spark Violet™ 500 anti-human CD19 HIB19 FC
PE/Fire™ 810 anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
Spark UV™ 387 anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Alexa Fluor® 660 anti-human CD19 HIB19 FC
PerCP/Fire™ 806 anti-human CD19 HIB19 FC
PerCP/Fire™ 780 anti-human CD19 HIB19 FC
Spark PLUS UV395™ anti-human CD19 HIB19 FC
Spark Red™ 718 anti-human CD19 HIB19 FC
FITC anti-human CD19 HIB19 FC
Go To Top Version: 2    Revision Date: 12/18/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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