- Clone
- HIR2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VII 70299
- Other Names
- Glycophorin A/B, GPA/GPB, GYPA, GYPB
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HIR2_PECy7_CD235ab_Antibody_090517 HIR2_PECy7_CD235ab_Antibody_090517](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HIR2_PECy7_CD235ab_Antibody_090517.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human erythrocytes were first treated with Human TruStain FcX™ then stained with clone HIR2 PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype control (dashed histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
306619 | 25 µg | £101 | ||||
306620 | 100 µg | £221 |
The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.02 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
- Product Citations
-
- RRID
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AB_2716163 (BioLegend Cat. No. 306619)
AB_2716164 (BioLegend Cat. No. 306620)
Antigen Details
- Structure
- Sialoglycoprotein, 20 kD
- Distribution
-
Erythrocytes
- Cell Type
- Erythrocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
- Gene ID
- 2993 View all products for this Gene ID
- UniProt
- View information about CD235ab on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD235ab Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-human CD235ab | HIR2 | FC |
PE anti-human CD235ab | HIR2 | FC |
PE/Cyanine5 anti-human CD235ab | HIR2 | FC |
Purified anti-human CD235ab | HIR2 | FC,CyTOF®,IHC-P,SB |
FITC anti-human CD235ab | HIR2 | FC |
Pacific Blue™ anti-human CD235ab | HIR2 | FC |
PerCP/Cyanine5.5 anti-human CD235ab | HIR2 | FC |
Purified anti-human CD235ab (Maxpar® Ready) | HIR2 | FC,CyTOF® |
Biotin anti-human CD235ab | HIR2 | FC |
PE/Cyanine7 anti-human CD235ab | HIR2 | FC |
APC/Fire™ 750 anti-human CD235ab | HIR2 | FC |
TotalSeq™-A0196 anti-human CD235ab | HIR2 | PG |
TotalSeq™-C0196 anti-human CD235ab | HIR2 | PG |
TotalSeq™-B0196 anti-human CD235ab | HIR2 | PG |
TotalSeq™-D0196 anti-human CD235ab | HIR2 | PG |
TotalSeq™-Bn0196 anti-human CD235ab | HIR2 | SB |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD235ab
Human red blood cells stained with HIR2 APC -
PE anti-human CD235ab
Human red blood cells stained with CD235a/b PE -
PE/Cyanine5 anti-human CD235ab
Human red blood cells stained with HIR2 PE/Cyanine5 -
Purified anti-human CD235ab
Human red blood cells stained with CD235a/b PE IHC staining of purified anti-human CD235ab antibody (clone ... SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
FITC anti-human CD235ab
Human peripheral red blood cells stained with HIR2 FITC -
Pacific Blue™ anti-human CD235ab
Human erythrocytes stained with HIR2 Pacific Blue™ -
PerCP/Cyanine5.5 anti-human CD235ab
Human red blood cells were stained with anti-human CD235ab ... -
Purified anti-human CD235ab (Maxpar® Ready)
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Biotin anti-human CD235ab
Human peripheral blood red cells were stained with biotinyla... -
PE/Cyanine7 anti-human CD235ab
Human erythrocytes were first treated with Human TruStain Fc... -
APC/Fire™ 750 anti-human CD235ab
Human erythrocytes were first treated with Human TruStain Fc... -
TotalSeq™-A0196 anti-human CD235ab
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TotalSeq™-C0196 anti-human CD235ab
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TotalSeq™-B0196 anti-human CD235ab
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TotalSeq™-D0196 anti-human CD235ab
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TotalSeq™-Bn0196 anti-human CD235ab
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