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PE/Cyanine7 anti-mouse CD309 (VEGFR2, Flk-1) Antibody

Pricing & Availability
Clone
Avas12 (See other available formats)
Regulatory Status
RUO
Other Names
Fetal liver kinase 1 (Flk-1), VEGF receptor-2, VEGF-R2, Ly-73, KDR
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
Avas12_PECy7_CD309_Antibody_FC_092012
bEND.3 mouse endothelial cells were stained with CD309 (clone Avas12) PE/Cyanine7 (filled histogram) or rat IgG2a, κ PE/Cyanine7 isotype control (open histogram).
  • Avas12_PECy7_CD309_Antibody_FC_092012
    bEND.3 mouse endothelial cells were stained with CD309 (clone Avas12) PE/Cyanine7 (filled histogram) or rat IgG2a, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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136413 25 µg £105
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136414 100 µg £230
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Description

CD309 is also known as vascular endothelial growth factor receptor 2 (VEGFR2) and fetal liver kinase-1 (Flk-1). CD309 is a member of the tyrosine protein kinase family that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. CD309 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle. It's a receptor for VEGF or VEGFC, and plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine Flk1 fused to hIgG Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Avas12 recognizes a different epitope than clone 89B3A5. Additional reported applications (for the relevant formats) include: Western blotting1 and immunohistochemical staining of paraformaldehyde-fixed frozen sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729. (WB)
  2. Ishitobi H, et al. 2010. Exp. Anim. 59:615. (IHC-F)
Product Citations
  1. Shen Z, et al. 2023. iScience. 26:106572. PubMed
  2. Hong SP, et al. 2019. World J Stem Cells. 11:44. PubMed
  3. Christoforou N, et al. 2013. PLoS One. 8:65963. PubMed
  4. Clark SJ, et al. 2022. Genome Biol. 23:202. PubMed
RRID
AB_2561606 (BioLegend Cat. No. 136413)
AB_2561606 (BioLegend Cat. No. 136414)

Antigen Details

Structure
A receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF receptor α chain) of the immunoglobulin superfamily
Distribution

Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle

Function
Plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability
Ligand/Receptor
Vascular endothelial growth factor (VEGF)
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Kaburn N, et al. 1997. Development. 124:2039
2. Patterson C, et al. 1995. J. Bio. Chem. 270:23111
3. Nishikawa SI, et al. 1998. Immunity 8 (6):761
4. Shalaby F, et al. 1997. Cell. 89 (6):981

Gene ID
16542 View all products for this Gene ID
UniProt
View information about CD309 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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