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PE/Cyanine7 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_PECy7_011909.jpg
TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 PE/Cyanine7
  • G8dot8_PECy7_011909.jpg
    TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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118215 25 µg £85
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118216 100 µg £221
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Buechler MB, et al. 2021. Nature. 593:575. PubMed
  2. Kozuki S, et al. 2022. Genes Cells. 27:192. PubMed
  3. Jeon HY, et al. 2022. EMBO J. 41:e109365. PubMed
  4. Weiner AI 2022. Cell Reports. 41(11):111805. PubMed
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  6. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
  7. Zhou B, et al. 2018. J Clin Invest. 7:e1438105. PubMed
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  9. Li D, et al. 2023. Sci Adv. 9:eadc9446. PubMed
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  12. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  13. Nayar S, et al. 2016. J Immunol. 197: 1957 - 1967. PubMed
  14. Teisanu R, et al. 2010. Am J Respir Cell Mol Biol. 44:794. PubMed
  15. Mourao L, et al. 2019. Sci Rep. 0.991666667. PubMed
  16. Ohigashi I, et al. 2021. Cell Rep. 36:109657. PubMed
  17. Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed
  18. Jahan S, et al. 2022. J Biol Chem. :102386. PubMed
  19. Scott CL et al. 2018. Immunity. 49(2):312-325 . PubMed
  20. Morales RA, et al. 2022. Mucosal Immunol. 15:940. PubMed
  21. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  22. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  23. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  24. Cen B, et al. 2021. Oncogene. 40:5984. PubMed
  25. Ohigashi I, et al. 2020. Cell Reports. 29(9):2901-2916.e6.. PubMed
  26. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  27. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  28. Sheahan BJ, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:119. PubMed
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  30. Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed
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  35. Kang MH, et al. 2020. Nat Commun. 3.186805556. PubMed
  36. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  37. Shanahan MT, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321:G668. PubMed
  38. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  39. Janela B, et al. 2019. Immunity. 50:1069. PubMed
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  41. McNeil M, et al. 2021. J Mammary Gland Biol Neoplasia. 26:357. PubMed
  42. Pietzner J, et al. 2012. Hum Mol Genet. 122:493. PubMed
  43. Dost AFM, et al. 2020. Cell Stem Cell. 27(4):663-678.e8. PubMed
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  50. Gonzalez M, et al. 2014. Proc Natl Acad Sci U S A. 111:3098. PubMed
  51. Legrand JMD, et al. 2019. Nat Commun. 10:2278. PubMed
  52. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  53. Louie SM, et al. 2022. Cell Rep. 39:110662. PubMed
  54. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed
  55. Kearns N, et al. 2013. Stem Cell Res. 11:1003. PubMed
  56. Nigro G, et al. 2014. Cell Host Microbe. 15:792. PubMed
  57. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  58. Napoli M, et al. 2022. Nat Commun. 13:614. PubMed
  59. Han Y, et al. 2022. Cell Rep. 38:110240. PubMed
  60. Nagatake T, et al. 2014. PLoS One. 9:90638. PubMed
  61. Liu B, et al. 2013. Development. 140:780. PubMed
  62. Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed
  63. Wang J, et al. 2022. Nat Protoc. 17:1359. PubMed
  64. Choi J, et al. 2020. Cell Stem Cell. 27:366. PubMed
  65. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  66. Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed
  67. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  68. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
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  71. Liang Z, et al. 2017. Autophagy. 14:505. PubMed
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  73. Wang D, et al. 2020. Cell. 1198:180. PubMed
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RRID
AB_1236471 (BioLegend Cat. No. 118215)
AB_1236471 (BioLegend Cat. No. 118216)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD326 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,ICC
Biotin anti-mouse CD326 (Ep-CAM) G8.8 FC
PE anti-mouse CD326 (Ep-CAM) G8.8 FC
FITC anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC,SB
PE/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
APC/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM) G8.8 ICC,IHC-F,3D IHC
Brilliant Violet 421™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 605™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) (Maxpar® Ready) G8.8 FC,CyTOF®
APC/Fire™ 750 anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 711™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 510™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Dazzle™ 594 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-A0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Alexa Fluor® 700 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-C0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 785™ anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-B0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 650™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Cyanine5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Spark Red™ 718 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 574 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 550 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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