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PE/Cyanine7 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_PECy7_011909.jpg
TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 PE/Cyanine7
  • G8dot8_PECy7_011909.jpg
    TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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118215 25 µg £85
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118216 100 µg £221
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
  2. Zhou B, et al. 2018. J Clin Invest. 7:e1438105. PubMed
  3. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  4. Buechler MB, et al. 2021. Nature. 593:575. PubMed
  5. Kozuki S, et al. 2022. Genes Cells. 27:192. PubMed
  6. Jeon HY, et al. 2022. EMBO J. 41:e109365. PubMed
  7. Weiner AI 2022. Cell Reports. 41(11):111805. PubMed
  8. Wei J, et al. 2022. Cancer Prev Res (Phila). 15:285. PubMed
  9. Li D, et al. 2023. Sci Adv. 9:eadc9446. PubMed
  10. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  11. Nayar S, et al. 2016. J Immunol. 197: 1957 - 1967. PubMed
  12. Teisanu R, et al. 2010. Am J Respir Cell Mol Biol. 44:794. PubMed
  13. Mourao L, et al. 2019. Sci Rep. 0.991666667. PubMed
  14. Soleimany AP, et al. 2022. Nat Commun. 13:5745. PubMed
  15. Fujimori S, et al. 2022. Elife. 11:. PubMed
  16. Scott CL et al. 2018. Immunity. 49(2):312-325 . PubMed
  17. Ohigashi I, et al. 2021. Cell Rep. 36:109657. PubMed
  18. Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed
  19. Jahan S, et al. 2022. J Biol Chem. :102386. PubMed
  20. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  21. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  22. Morales RA, et al. 2022. Mucosal Immunol. 15:940. PubMed
  23. Ohigashi I, et al. 2020. Cell Reports. 29(9):2901-2916.e6.. PubMed
  24. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  25. Cen B, et al. 2021. Oncogene. 40:5984. PubMed
  26. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  27. Osborne JK, et al. 2021. Cell Reports. 36(3):109408. PubMed
  28. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  29. Sheahan BJ, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:119. PubMed
  30. Kass E, et al. 2016. Nat Commun. 7:13241. PubMed
  31. Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed
  32. Lee JH et al. 2017. Cell. 170(6):1149-1163 . PubMed
  33. Dar HH, et al. 2022. JCI Insight. 7:. PubMed
  34. Kang MH, et al. 2020. Nat Commun. 3.186805556. PubMed
  35. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  36. Sun P, et al. 2021. J Mammary Gland Biol Neoplasia. 26:377. PubMed
  37. Shanahan MT, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321:G668. PubMed
  38. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  39. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  40. Tu WL, et al. 2018. Am J Pathol. 188:2688. PubMed
  41. Pietzner J, et al. 2012. Hum Mol Genet. 122:493. PubMed
  42. Dost AFM, et al. 2020. Cell Stem Cell. 27(4):663-678.e8. PubMed
  43. Yip HYK, et al. 2018. PLoS One. 3. PubMed
  44. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  45. McNeil M, et al. 2021. J Mammary Gland Biol Neoplasia. 26:357. PubMed
  46. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  47. Gonzalez M, et al. 2014. Proc Natl Acad Sci U S A. 111:3098. PubMed
  48. Legrand JMD, et al. 2019. Nat Commun. 10:2278. PubMed
  49. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  50. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
  51. Galani IE, et al. 2022. STAR Protoc. 3:101151. PubMed
  52. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed
  53. Kearns N, et al. 2013. Stem Cell Res. 11:1003. PubMed
  54. Nigro G, et al. 2014. Cell Host Microbe. 15:792. PubMed
  55. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  56. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  57. Louie SM, et al. 2022. Cell Rep. 39:110662. PubMed
  58. Nagatake T, et al. 2014. PLoS One. 9:90638. PubMed
  59. Liu B, et al. 2013. Development. 140:780. PubMed
  60. Napoli M, et al. 2022. Nat Commun. 13:614. PubMed
  61. Han Y, et al. 2022. Cell Rep. 38:110240. PubMed
  62. Choi J, et al. 2020. Cell Stem Cell. 27:366. PubMed
  63. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  64. Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed
  65. Wang J, et al. 2022. Nat Protoc. 17:1359. PubMed
  66. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  67. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  68. Liang Z, et al. 2017. Autophagy. 14:505. PubMed
  69. Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed
  70. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  71. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  72. Liu X, et al. 2021. iScience. 24(6):102551. PubMed
  73. Wang D, et al. 2020. Cell. 1198:180. PubMed
  74. Jin H, et al. 2018. Am J Physiol Lung Cell Mol Physiol. 314:L882. PubMed
  75. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  76. Sun RC, et al. 2019. Cell Metab. 30:903. PubMed
  77. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
  78. Luo N, et al. 2018. Nat Commun. 0.547222222. PubMed
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RRID
AB_1236471 (BioLegend Cat. No. 118215)
AB_1236471 (BioLegend Cat. No. 118216)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD326 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,ICC
Biotin anti-mouse CD326 (Ep-CAM) G8.8 FC
PE anti-mouse CD326 (Ep-CAM) G8.8 FC
FITC anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC,SB
PE/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
APC/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM) G8.8 ICC,IHC-F,3D IHC
Brilliant Violet 421™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 605™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) (Maxpar® Ready) G8.8 FC,CyTOF®
APC/Fire™ 750 anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 711™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 510™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Dazzle™ 594 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-A0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Alexa Fluor® 700 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-C0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 785™ anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-B0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 650™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Cyanine5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Spark Red™ 718 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 574 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 550 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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