Purified anti-mouse CD326 Ep-CAM Maxpar Ready Antibody anti-CD326

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Clone: G8.8 (See other available formats)
Regulatory Status: RUO
Other Names: CD326, EGP40, MIC18, TROP1, KSA
Isotype: Rat IgG2a, κ
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Product Citations: publications

G8.8_Purified_CD326_Cytof_060414.jpg — Mouse TE-71 (top) or Chinese Hamster Ovary (CHO) (bottom) cells stained with 165Ho anti-CD326/EpCAM (G8.8). Data provided by DVS Sciences.

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118223

100 µg

£85

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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: TE-71 thymic epithelial cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed¹, with or without OCT embedding^2,4, and spatial biology (IBEX)^13,14.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563743 (BioLegend Cat. No. 118223)

Antigen Details

Structure: 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution: Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.
Function: Mediates calcium-independent homophilic cell-cell adhesion.
Interaction: CD326 displays hemophilic binding.
Ligand/Receptor: CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type: Embryonic Stem Cells, Epithelial cells
Biology Area: Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.
Gene ID: 17075 View all products for this Gene ID
UniProt: View information about CD326 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD326 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD326 (Ep-CAM)	G8.8	FC
Purified anti-mouse CD326 (Ep-CAM)	G8.8	FC,IHC-F,ICC
Biotin anti-mouse CD326 (Ep-CAM)	G8.8	FC
PE anti-mouse CD326 (Ep-CAM)	G8.8	FC
FITC anti-mouse CD326 (Ep-CAM)	G8.8	FC
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM)	G8.8	FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM)	G8.8	FC,IHC-F,3D IHC,SB
PE/Cyanine7 anti-mouse CD326 (Ep-CAM)	G8.8	FC
APC/Cyanine7 anti-mouse CD326 (Ep-CAM)	G8.8	FC
PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM)	G8.8	FC
Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM)	G8.8	ICC,IHC-F,3D IHC
Brilliant Violet 421™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
Brilliant Violet 605™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
Purified anti-mouse CD326 (Ep-CAM) (Maxpar® Ready)	G8.8	FC,CyTOF®
APC/Fire™ 750 anti-mouse CD326 (Ep-CAM)	G8.8	FC
Brilliant Violet 711™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
Brilliant Violet 510™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
PE/Dazzle™ 594 anti-mouse CD326 (Ep-CAM)	G8.8	FC
TotalSeq™-A0449 anti-mouse CD326 (Ep-CAM)	G8.8	PG
Alexa Fluor® 700 anti-mouse CD326 (Ep-CAM)	G8.8	FC
TotalSeq™-C0449 anti-mouse CD326 (Ep-CAM)	G8.8	PG
Brilliant Violet 785™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
TotalSeq™-B0449 anti-mouse CD326 (Ep-CAM)	G8.8	PG
Brilliant Violet 650™ anti-mouse CD326 (Ep-CAM)	G8.8	FC
PE/Cyanine5 anti-mouse CD326 (Ep-CAM)	G8.8	FC
Spark Red™ 718 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™)	G8.8	FC
Spark Blue™ 574 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™)	G8.8	FC
Spark Blue™ 550 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™)	G8.8	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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