Loading...

Purified anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M5/114.15.2
C57/B6 mouse splenocytes were stained with purified anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or purified rat IgG2b, κ isotype control (dashed line) followed by IgG-FITC.
  • M5/114.15.2
    C57/B6 mouse splenocytes were stained with purified anti-mouse I-A/I-E (clone M5/114.15.2) (solid line) or purified rat IgG2b, κ isotype control (dashed line) followed by IgG-FITC.
  • M5slash114_Pure_I-A-I-E_Antibody_102518
    Fresh, frozen mouse spleen was stained with purified I-A/I-E clone M5/114.15.2 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with F4/80 Cy5 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Compare all formats
Cat # Size Price Quantity Check Availability Save
107601 50 µg £45
Check Availability


Need larger quantities of this item?
Request Bulk Quote
107602 500 µg £173
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  2. Zaman R, et al. 2021. Immunity. :. PubMed
  3. Farkas AM, et al. 2019. J Allergy Clin Immunol. 143:335. PubMed
  4. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  5. Sabatino J, et al. 2011. J Exp Med. 208:81. PubMed
  6. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  7. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  8. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  9. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  10. Campisi L, et al. 2022. Nature. 606:945. PubMed
  11. Han G, et al. 2022. Gut Microbes. 14:2104086. PubMed
  12. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  13. Liu HJ, et al. 2023. Nat Commun. 14:1214. PubMed
  14. Lau SF, et al. 2020. Cell Rep. 31:107530. PubMed
  15. Sorrelle N, et al. 2019. J Immunol. 202:292. PubMed
  16. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  17. Jung J, et al. 2019. Cell Host Microbe. 25:513. PubMed
  18. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  19. Perez-Muñoz M, et al. 2015. PLoS One. 10: 0144382. PubMed
  20. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  21. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  22. Sparber F, et al. 2018. PLoS Pathog. 14:e1007069. PubMed
  23. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  24. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  25. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  26. Bonnefoy F, et al. 2018. Front Immunol. 2.170833333. PubMed
  27. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  28. Mayer JU, et al. 2020. Front Immunol. 11:592325. PubMed
  29. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  30. Fiala GJ, et al. 2019. J Immunol. 203:569. PubMed
  31. Claser C, et al. 2019. Nat Commun. 3.361805556. PubMed
  32. Mouat IC, et al. 2021. Elife. 10:. PubMed
  33. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  34. Kuns R, et al. 2009. Blood. 113:5999. PubMed
  35. Hitchcock JR, et al. 2020. FEBS J. 287:250. PubMed
  36. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  37. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  38. Yang M, et al. 2020. Oncoimmunology. 9:1708064. PubMed
  39. Toulmin SA, et al. 2021. Nat Commun. 12:3993. PubMed
  40. Park S, et al. 2021. Nat Cell Biol. 23:476. PubMed
  41. Zhao J, et al. 2012. J Virol. 86:11416. PubMed
  42. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  43. Hausmann A, et al. 2021. J Exp Med. 218:. PubMed
  44. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  45. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  46. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  47. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  48. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  49. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
  50. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  51. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  52. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  53. Wei S, et al. 2019. Virulence. 0.588888889. PubMed
  54. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  55. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  56. Enders M, et al. 2020. J Immunol. 204:87. PubMed
  57. Dai Z, et al. 2021. JCI Insight. 6: . PubMed
  58. Ho NI, et al. 2021. Immunology. 164:494. PubMed
  59. Krueger A, et al. 2022. Front Microbiol. 12:789042. PubMed
  60. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  61. Matte-Martone C, et al. 2008. Blood. 111:3884. PubMed
  62. Pascalis R, et al. 2008. Infect Immun. 76:4331. PubMed
  63. Luckashenak N, et al. 2006. J Immunol. 177:5177. PubMed
  64. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  65. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  66. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  67. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  68. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  69. Hoffmann FM, et al. 2018. Front Immunol. 9:1046. PubMed
  70. Li J, et al. 2021. Front Immunol. 12:710406. PubMed
  71. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  72. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
  73. Grubiši? V, et al. 2020. Cell Rep. 32:108100. PubMed
  74. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  75. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  76. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  77. Heyman O, et al. 2018. Front Immunol. 1.563888889. PubMed
  78. Zhang J, et al. 2019. J Clin Invest. 130:. PubMed
  79. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  80. Li J, et al. 2020. Development. :. PubMed
  81. Jux B, et al. 2013. Int Immunol. 25:307. PubMed
  82. König S, et al. 2014. PLoS One. 9:93555. PubMed
  83. Hacini–Rachinel F, et al. 2018. J Allergy Clin Immunol. 141:163. PubMed
  84. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  85. Inclan-Rico JM, et al. 2020. PLoS Pathog. 16:e1008579. PubMed
  86. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  87. Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
  88. Phan TS, et al. 2021. Sci Adv. 7:. PubMed
  89. Ipseiz N, et al. 2020. EMBO J. 39:e103454. PubMed
  90. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  91. Lee MC, et al. 2017. J Immunol. 198:3565. PubMed
  92. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  93. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  94. Chen B, et al. 2021. Cell. 184:6262. PubMed
  95. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  96. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  97. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  98. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  99. Schneppenheim J, et al. 2017. J Immunol. 199:172. PubMed
  100. Zaric M, et al. 2017. J Control Release. 268:166. PubMed
  101. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  102. Hassel C, et al. 2021. Front Immunol. 12:754661. PubMed
  103. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  104. Wu W, et al. 2017. Sci Rep. 7:44481. PubMed
  105. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  106. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
RRID
AB_313316 (BioLegend Cat. No. 107601)
AB_313316 (BioLegend Cat. No. 107602)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 5    Revision Date: 10/25/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account