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PE/Dazzle™ 594 anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
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Product Citations
publications
X54-5slash7.1_PEDZL594_CD64_Antibody_FC_1_102715
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) APC and CD64 (clone X54-5/7.1) PE/Dazzle™ 594 (top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (bottom).
  • X54-5slash7.1_PEDZL594_CD64_Antibody_FC_1_102715
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) APC and CD64 (clone X54-5/7.1) PE/Dazzle™ 594 (top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (bottom).
  • X54-5slash7.1_PEDZL594_CD64_Antibody_FC_2_102715
Compare all formats See PE/Dazzle™ 594 spectral data
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139319 25 µg £109
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139320 100 µg £254
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  2. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  3. Sepe JJ, et al. 2022. JACC Basic Transl Sci. 7:915. PubMed
  4. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  5. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  6. Marangoni F, et al. 2021. Cell. . PubMed
  7. Lu S, et al. 2020. JCI Insight. 5:00. PubMed
  8. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  9. Forbester JL, et al. 2020. J Virol. 94:. PubMed
  10. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  11. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  12. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  13. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
RRID
AB_2566558 (BioLegend Cat. No. 139319)
AB_2566558 (BioLegend Cat. No. 139320)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org
Go To Top Version: 2    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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