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PerCP/Cyanine5.5 anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
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Product Citations
publications
X545-71_PerCPCyanine55_CD64_Antibody_1_030624
C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on myeloid population.
  • X545-71_PerCPCyanine55_CD64_Antibody_1_030624
    C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on myeloid population.
Compare all formats See PerCP/Cyanine5.5 spectral data
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139307 25 µg £121
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139308 100 µg £262
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Maccio-Maretto L, et al. 2022. Immunology. 167:64. PubMed
  2. Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed
  3. Caslin HL, et al. 2023. Front Immunol. 13:984859. PubMed
  4. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  5. van Wijck Y, et al. 2018. J Immunol. 200:1570. PubMed
  6. Vaillancourt M, et al. 2023. PLoS Pathog. 19:e1010942. PubMed
  7. Pisu D, et al. 2020. STAR Protoc. 1:100123. PubMed
  8. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  9. Dodagatta-Marri E, et al. 2021. Cell Rep. 36:109309. PubMed
  10. De Schepper S et al. 2018. Cell. 175(2):400-415 . PubMed
  11. Marinaik CB, et al. 2020. Cell Rep Med. 1:100095. PubMed
  12. Baasch S, et al. 2021. Cell. . PubMed
  13. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  14. Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed
  15. Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed
  16. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  17. Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed
  18. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  19. Pisu D, et al. 2020. Cell Rep. 30:335. PubMed
  20. Lee W, et al. 2021. J Virol. 95:e0053021. PubMed
  21. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  22. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  23. Shibata T, et al. 2020. J Clin Invest. 130:3021. PubMed
  24. Sung PS, et al. 2022. JCI Insight. :. PubMed
  25. Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed
  26. Hakim R, et al. 2021. J Neurosci. 41:8441. PubMed
  27. Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed
  28. Feuerstein R, et al. 2015. J Immunol. 194:2735. PubMed
  29. Kolter J, et al. 2016. J Immunol. 196: 2733 - 2741. PubMed
  30. Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed
  31. Subramanian S, et al. 2022. Nat Immunol. 23:458. PubMed
  32. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  33. Ge Y, et al. 2020. Mucosal Immunol. 13:34. PubMed
  34. Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed
RRID
AB_2561962 (BioLegend Cat. No. 139307)
AB_2561962 (BioLegend Cat. No. 139308)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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