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PE/Cyanine7 anti-human CD178 (FasL) Antibody

Pricing & Availability
Clone
NOK-1 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70322
Other Names
Fas Ligand (FasL), CD95L, TNFSF6, Fas-L
Isotype
Mouse IgG1, κ
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Product Citations
publications
NOK-1_PECyanine7_CD178_Antibody_080519
Human Fas Ligand transfected cells were stained with CD178 (Fas-L) (clone NOK-1) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
  • NOK-1_PECyanine7_CD178_Antibody_080519
    Human Fas Ligand transfected cells were stained with CD178 (Fas-L) (clone NOK-1) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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306417 25 tests £113
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306418 100 tests £230
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Description

CD178 is a 38-42 kD type II glycoprotein also known as Fas ligand and CD95L. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
L5178Y mouse T lymphoma cells expressing recombinant human FasL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, immunofluorescence microscopy3, immunocytochemistry2, blocking of Fas induced apoptosis1, and Western blotting11. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306415 and 306416).

Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
  2. Herr I, et al. 2000. Cell Death Differ. 7:129. (WB)
  3. Bossi G, et al. 1999. Nature Medicine 5:90.
  4. Andreola G, et al. 2002. J. Exp. Med. 195:1303.
  5. Strauss L, et al. 2009. J. Immunol. 182:1469. PubMed
  6. Li JH, et al. 2009. Am J. Pathol. 175:1124. PubMed
  7. Zhao Q, et al. 2011. Fitoterapia. 82:735. PubMed
  8. Kruger K, et al. 2011. J. Appl. Physiol. 110:1226. PubMed
  9. Qin G, et al. 2012. J. Infect. Dis. PubMed
  10. Khalid M, et al. 2012. J. Virol. 86:4906. PubMed
  11. Qin G, et al. 2012. J. Infect. Dis. 205:1646. PubMed
  12. Shrestha B, et al. 2012. J. Virol. 86:8937. PubMed
  13. Mooren FC, et al. 2012. J. Appl. Physiol.113:1082. PubMed
  14. Robinet P, et al. 2014. J Immunol. 192:5332. PubMed
  15. Wang Y, et al. 2014. J Endocrinol. 222:151. PubMed
Product Citations
  1. Lanna A, et al. 2022. Nat Cell Biol. 24:1461. PubMed
RRID
AB_2814147 (BioLegend Cat. No. 306417)
AB_2814147 (BioLegend Cat. No. 306418)

Antigen Details

Structure
TNF superfamily, type II glycoprotein, 38-42 kD, 26 kD soluble form
Distribution

Activated T cells, NK cells, testis, eye, neutrophils, Clara type II cells

Function
Apoptosis, immune privilege
Ligand/Receptor
CD95
Cell Type
Neutrophils, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Suda T, et al. 1997. J. Exp. Med. 12:204.
2. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
3. Tanaka M, et al. 1995. EMBO J. 14:1129.

Gene ID
356 View all products for this Gene ID
UniProt
View information about CD178 on UniProt.org
Go To Top Version: 1    Revision Date: 08/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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