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PerCP anti-human CD11a Antibody

Pricing & Availability
Clone
TS2/4 (See other available formats)
Regulatory Status
RUO
Workshop
V S160
Other Names
LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TS2slash4_PerCP_100610
Human peripheral blood lymphocytes stained with PerCP anti-human CD11a (clone TS2/4, filled histogram) or PerCP mouse IgG1, κ isotype control (open histogram)
  • TS2slash4_PerCP_100610
    Human peripheral blood lymphocytes stained with PerCP anti-human CD11a (clone TS2/4, filled histogram) or PerCP mouse IgG1, κ isotype control (open histogram)
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350608 100 tests £182
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Description

CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HLA-DR CTL line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1, 2. Note: the antibody TS2/4 recognizes the ß-propeller domain of CD11a and only immunoprecipitates the CD11a/CD18 complex.

Application References

(PubMed link indicates BioLegend citation)
  1. Huang C and Springer TA. 1997. P. Natl. Acad. Sci. USA 94:3162. (IP)
  2. Sanchez-Madrid F, et al. 1983. J. Exp. Med. 158:1785. (IP)
  3. Sanchez-Madrid F, et al. 1982. P. Natl. Acad. Sci. USA 79:7489. (FC)
Product Citations
  1. Friede ME, et al. 2018. J Immunol. 983:200. PubMed
  2. Wang Y, et al. 2019. Adv Sci (Weinh). 1901278:6. PubMed
  3. Mancuso RV, et al. 2020. British Journal of Pharmacology. 177(12):2696-2711.. PubMed
RRID
AB_10662249 (BioLegend Cat. No. 350608)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β2 (CD18) forms LFA-1, 170-180 kD
Distribution

Leukocytes

Function
Adhesion, costimulation
Ligand/Receptor
ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146.

Gene ID
3683 View all products for this Gene ID
UniProt
View information about CD11a on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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